世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
5期
813-817
,共5页
张涛%吉婧%纪恩茹%陈斌%黄裕红
張濤%吉婧%紀恩茹%陳斌%黃裕紅
장도%길청%기은여%진빈%황유홍
HBV相关性肝衰竭%阴黄证%阳黄证%树突状细胞
HBV相關性肝衰竭%陰黃證%暘黃證%樹突狀細胞
HBV상관성간쇠갈%음황증%양황증%수돌상세포
HBV-related liver failure%Y in-Huang syndrome%Y ang-Huang syndrome%dendritic cells
目的：比较乙型肝炎（HBV）相关性肝衰竭阴黄证、阳黄证患者外周血树突状细胞（DCs）功能，研究阴黄证、阳黄证两证型DCs的免疫表达特点。方法：将HBV相关性肝衰竭黄疸症患者分为阳黄组与阴黄组两组，另设健康对照组，以外周血来源的PBMCs体外分离诱导培养DCs，应用流式细胞计数检测DCs的表面分子HLA-DR、CD80、CD86、CD83、CD1α，并检测DCs的上清液中IFN-α、IL-4的分泌水平，比较HBV相关性肝衰竭患者阴、阳黄证与免疫细胞及炎症因子表达的差异。结果：与健康组比较，HBV相关性肝衰竭患者DCs表型HLA-DR、CD1α、CD83、CD80、CD86表达率显著下降（P<0.01）， DCs分泌因子IFN-α显著升高（P<0.01）；与阳黄组比较，HBV相关性肝衰竭患者阴黄组CD83、CD86表达率显著降低（P<0.01），IL-4表达显著增强（P<0.01）；与阴黄组比较，阳黄证组DCs分泌因子IFN-α表达显著增强（P<0.01）。结论：阴黄、阳黄两组HBV相关性肝衰竭患者的DCs功能状态均表现为细胞免疫功能低下，阴黄证患者的细胞免疫功能更低，存在抗炎症细胞因子表达增强；阳黄证患者存在炎症因子过度释放。
目的：比較乙型肝炎（HBV）相關性肝衰竭陰黃證、暘黃證患者外週血樹突狀細胞（DCs）功能，研究陰黃證、暘黃證兩證型DCs的免疫錶達特點。方法：將HBV相關性肝衰竭黃疸癥患者分為暘黃組與陰黃組兩組，另設健康對照組，以外週血來源的PBMCs體外分離誘導培養DCs，應用流式細胞計數檢測DCs的錶麵分子HLA-DR、CD80、CD86、CD83、CD1α，併檢測DCs的上清液中IFN-α、IL-4的分泌水平，比較HBV相關性肝衰竭患者陰、暘黃證與免疫細胞及炎癥因子錶達的差異。結果：與健康組比較，HBV相關性肝衰竭患者DCs錶型HLA-DR、CD1α、CD83、CD80、CD86錶達率顯著下降（P<0.01）， DCs分泌因子IFN-α顯著升高（P<0.01）；與暘黃組比較，HBV相關性肝衰竭患者陰黃組CD83、CD86錶達率顯著降低（P<0.01），IL-4錶達顯著增彊（P<0.01）；與陰黃組比較，暘黃證組DCs分泌因子IFN-α錶達顯著增彊（P<0.01）。結論：陰黃、暘黃兩組HBV相關性肝衰竭患者的DCs功能狀態均錶現為細胞免疫功能低下，陰黃證患者的細胞免疫功能更低，存在抗炎癥細胞因子錶達增彊；暘黃證患者存在炎癥因子過度釋放。
목적：비교을형간염（HBV）상관성간쇠갈음황증、양황증환자외주혈수돌상세포（DCs）공능，연구음황증、양황증량증형DCs적면역표체특점。방법：장HBV상관성간쇠갈황달증환자분위양황조여음황조량조，령설건강대조조，이외주혈래원적PBMCs체외분리유도배양DCs，응용류식세포계수검측DCs적표면분자HLA-DR、CD80、CD86、CD83、CD1α，병검측DCs적상청액중IFN-α、IL-4적분비수평，비교HBV상관성간쇠갈환자음、양황증여면역세포급염증인자표체적차이。결과：여건강조비교，HBV상관성간쇠갈환자DCs표형HLA-DR、CD1α、CD83、CD80、CD86표체솔현저하강（P<0.01）， DCs분비인자IFN-α현저승고（P<0.01）；여양황조비교，HBV상관성간쇠갈환자음황조CD83、CD86표체솔현저강저（P<0.01），IL-4표체현저증강（P<0.01）；여음황조비교，양황증조DCs분비인자IFN-α표체현저증강（P<0.01）。결론：음황、양황량조HBV상관성간쇠갈환자적DCs공능상태균표현위세포면역공능저하，음황증환자적세포면역공능경저，존재항염증세포인자표체증강；양황증환자존재염증인자과도석방。
This study was aimed to compare the function of dendritic cells (DCs) in patients of Y in-Huang syn-drome and Y ang-Huang syndrome from the peripheral blood of HBV related hepatic failure. Jaundice patients of HBV related hepatic failure were divided into two groups, which were the Y ang-Huang syndrome group and Y in-Huang syndrome group. And a healthy control group was also established. The DCs were separated and cultured in vitro from PBMCs in the peripheral blood. Surface molecules such as HLA-DR, CD80, CD86, CD83 and CD1αwere detected by the flow cytometry. And the level of IFN- α and IL-4 in the supernatant of DCs were also de-tected. The expression difference of inflammatory cytokines and immune cells between Y in-Huang and Y ang-Huang syndrome in patients of HBV related hepatic failure were compared. The results showed that compared with healthy people, the expression rate of DCs phenotype such as HLA-DR, CD1α, CD83, CD80, CD86 in patients of HBV related hepatic failure was significantly decreased (P < 0.01); the excreted factor IFN-α had a significant rising (P < 0.01). Compared to Y ang-Huang syndrome group, the expression rate of CD83 and CD86 in the Y in-Huang syndrome group of HBV related hepatic failure patients was significantly reduced (P < 0.01). And the ex-creted factor of IL-4 had a significant rising in Y in-Huang syndrome group (P < 0.01). Compared to the Y in-Huang syndrome group, the excreted factor IFN-α in the Yang-Huang syndrome group had a significant rising (P< 0.01). It was concluded that DCs function of patients with HBV related hepatic failure in both Y in-Huang syn-drome and Y ang-Huang syndrome group showed low immune function. And the immune function of the Y in-Huang syndrome group was lower. There was excessive releasing of inflammatory factors in the Y ang-Huang syn-drome group. And there was enhanced anti-inflammatory cytokine expression in the Y in-Huang group.
