CAJ | 학술논문

目的研究肿瘤坏死因子-α（ TNF-α）与声门不同状态下闭合性胸部撞击所致肺损伤的关系。方法采用逆转录聚合酶链反应（ RT-PCR）法检测48只新西兰大白兔声门紧闭与（或）开放状态下，不同时相点伤侧肺组织TNF-αmRNA的表达，以6只正常新西兰大白兔为对照。结果在伤后2h时，声门紧闭与开放两组TNF-αmRNA的表达无显著差别（P＞0.05），伤后4、8、12h声门紧闭组TNF-αmRNA的表达显著高于声门开放组（ P＜0.05）。伤侧肺组织中TNF-αmRNA表达各时相点显著高于对照组（ P＜0.05）。结论TNF-α参与创伤性肺损伤的发生发展并起重要作用，声门紧闭状态下肺撞击伤的损伤程度重于声门开放状态。
목적연구종류배사인자-α（ TNF-α）여성문불동상태하폐합성흉부당격소치폐손상적관계。방법채용역전록취합매련반응（ RT-PCR）법검측48지신서란대백토성문긴폐여（혹）개방상태하，불동시상점상측폐조직TNF-αmRNA적표체，이6지정상신서란대백토위대조。결과재상후2h시，성문긴폐여개방량조TNF-αmRNA적표체무현저차별（P＞0.05），상후4、8、12h성문긴폐조TNF-αmRNA적표체현저고우성문개방조（ P＜0.05）。상측폐조직중TNF-αmRNA표체각시상점현저고우대조조（ P＜0.05）。결론TNF-α삼여창상성폐손상적발생발전병기중요작용，성문긴폐상태하폐당격상적손상정도중우성문개방상태。
Objective To study the relationship between TNF-αand lung injury induced by blunt chest trauma under different glottis states .Methods The TNF-αmRNA expression of the injured lung tissue in the 48 New Zealand rabbits was detected by reverse transcription polymerase chain reaction ( RT-PCR) under the closed and open glottis states at designed time .Meanwhile,6 healthy New Zealand rats were taken as control .Results There was no remarkable difference in the expressions of TNF-αof lung tissue between the closed glottis group and the open glottis group after 2 hours after injury(P>0.05).But the TNF-αmRNA expression was obviously higher in the closed glottis group than that in open glttis group at 4,8,and 12 hours after injury(P<0.05).TNF-αmRNA expressions were much higher in injured lung tissue than that in control group at designed time (P<0.05).Con-clusion TNF-αis involved in the occurrence and development of traumatic lung injuries and plays a critical role in this process .The severity of lung injuries under the closed glottis state is significantly higher than that under open glottis state .