CAJ | 학술논문

利用RT-PCR扩增并克隆了含人血清白蛋白序列长约1.8 kb的片段，酶切鉴定并进行序列分析，测序结果与GenBank中登录的人血清白蛋白cDNA序列的同源性为97%；以鹌鹑卵清蛋白5’调控区POV来启动HAS的表达。用限制性内切酶kpnI和HindⅢ双酶切质粒pOV和pHSA，然后用T4连接酶定向连接成重组质粒pOV-pHSA，经酶切鉴定，两者成功融合。
이용RT-PCR확증병극륭료함인혈청백단백서렬장약1.8 kb적편단，매절감정병진행서렬분석，측서결과여GenBank중등록적인혈청백단백cDNA서렬적동원성위97%；이암순란청단백5’조공구POV래계동HAS적표체。용한제성내절매kpnI화HindⅢ쌍매절질립pOV화pHSA，연후용T4련접매정향련접성중조질립pOV-pHSA，경매절감정，량자성공융합。
About 1.8 kb sequence of the fragment of human serum albumin containing were successfully amplified by RT-PCR.The purpose of the initia gene were identified with the help of electrophoresis. The DNA fragment was cloned into vector pGEM-T and transformed into E.coli DH5a host bacteria. Xba Iendonuclease digestion and sequencingfrom selected positive cloned bacteria. The fragment was sequenced,and it was compared with serum albumin cDNA gene of humanin GenBank.The results indicated the homology were 97%.Using 5’-flanking of ovalbumin in quail for the control sequence to activate the expression of HAS.With restrictive interior contact enzyme kpnI with HindⅢ cuts material particle pOV and pHSA,then connects reorganization material particle for pOV-pHSA with the T4 ligase. The fusion vector was sequenced by enzyme digestion.