中西医结合心血管病电子杂志
中西醫結閤心血管病電子雜誌
중서의결합심혈관병전자잡지
Cardiovascular Disease Journal of Integrated Traditional Chinese and Western Medicine (Electronic)
2014年
2期
1-2
,共2页
赵文珍%何颖红%王云涛%杨勇琴
趙文珍%何穎紅%王雲濤%楊勇琴
조문진%하영홍%왕운도%양용금
SD大鼠%附睾上皮%原代细胞培养
SD大鼠%附睪上皮%原代細胞培養
SD대서%부고상피%원대세포배양
SD rat%The epididymal epithelium%Primary cell culture
目的:为获得生长状态佳,精子少的附睾上皮原代培养细胞。方法采取两种方法,取SD大鼠附睾上皮组织,均以酶消化为主,制成细胞悬液,进行培养。结果方法一:制成的细胞悬液中有较多的精子存在,培养的细胞中成纤维细胞占较大比例;方法二:培养结果比较好,精子少或无,培养的细胞中绝大部分为上皮细胞,且生长速度较快。结论获得生长状态佳,精子少的附睾上皮原代培养细胞,为研究附睾上皮中与精子功能成熟和受精相关的关键分子功能提供了必要的基础和条件。
目的:為穫得生長狀態佳,精子少的附睪上皮原代培養細胞。方法採取兩種方法,取SD大鼠附睪上皮組織,均以酶消化為主,製成細胞懸液,進行培養。結果方法一:製成的細胞懸液中有較多的精子存在,培養的細胞中成纖維細胞佔較大比例;方法二:培養結果比較好,精子少或無,培養的細胞中絕大部分為上皮細胞,且生長速度較快。結論穫得生長狀態佳,精子少的附睪上皮原代培養細胞,為研究附睪上皮中與精子功能成熟和受精相關的關鍵分子功能提供瞭必要的基礎和條件。
목적:위획득생장상태가,정자소적부고상피원대배양세포。방법채취량충방법,취SD대서부고상피조직,균이매소화위주,제성세포현액,진행배양。결과방법일:제성적세포현액중유교다적정자존재,배양적세포중성섬유세포점교대비례;방법이:배양결과비교호,정자소혹무,배양적세포중절대부분위상피세포,차생장속도교쾌。결론획득생장상태가,정자소적부고상피원대배양세포,위연구부고상피중여정자공능성숙화수정상관적관건분자공능제공료필요적기출화조건。
Objective To obtain good growth state,less sperm epididymal epithelial cells in primary culture.Methods Adopt two kinds of methods.The epididymal epithelial tissues of SD rats were mainly made by enzyme digestion,were made cell suspension and were cul-tured.Results Cultured fibroblasts accounted for a large proportion from the first method;The culture results from the second method are good,the sperm less or no,cultured cells for the vast majority of epithelial cells,and the growth speed.Conclusion To obtain good growth state conclusion,provides the basis and conditions necessary for the study of epididymis and sperm function maturation and fertilization of the key molecules associated function.