CAJ | 학술논문

为研究环境因子在厚壳贻贝(Mytilus coruscus)稚贝附着过程中的调控作用，作者探讨了低盐度对微生物膜生物构成、群落结构的影响及所形成微生物膜对厚壳贻贝稚贝附着的影响。在实验室条件下，研究微生物膜的日龄与干质量、细菌密度和硅藻密度、叶绿素 a 含量的关系及其对厚壳贻贝稚贝附着的影响。通过DGGE指纹图谱技术对微生物膜中的细菌群落结构多样性进行了分析。研究发现，盐度13和23时形成的微生物膜能有效促进厚壳贻贝稚贝的附着，且盐度23、28 d时稚贝附着率最高，达到72%。相关性分析表明，微生物膜的诱导活性与盐度、干质量、细菌密度、硅藻密度、日龄呈显著正相关性，与叶绿素 a 无相关性。微生物膜的干质量、附着细菌密度及底栖硅藻密度明显随着日龄的增加而增加，叶绿素 a 含量与微生物膜日龄无显著相关性。细菌群落在厚壳贻贝稚贝附着过程中发挥重要调控作用。
위연구배경인자재후각이패(Mytilus coruscus)치패부착과정중적조공작용，작자탐토료저염도대미생물막생물구성、군락결구적영향급소형성미생물막대후각이패치패부착적영향。재실험실조건하，연구미생물막적일령여간질량、세균밀도화규조밀도、협록소 a 함량적관계급기대후각이패치패부착적영향。통과DGGE지문도보기술대미생물막중적세균군락결구다양성진행료분석。연구발현，염도13화23시형성적미생물막능유효촉진후각이패치패적부착，차염도23、28 d시치패부착솔최고，체도72%。상관성분석표명，미생물막적유도활성여염도、간질량、세균밀도、규조밀도、일령정현저정상관성，여협록소 a 무상관성。미생물막적간질량、부착세균밀도급저서규조밀도명현수착일령적증가이증가，협록소 a 함량여미생물막일령무현저상관성。세균군락재후각이패치패부착과정중발휘중요조공작용。
The influence of low salinity formed biofilms on the settlement of the plantigrade Mytilus coruscus was investigated in the laboratory. Various aspects of biofilms were examined including age, dry weight, chlorophyll a concentration, bacterial and diatom densitie. The diversity of bacterial community structure was analyzed by fin-gerprint technique DGGE. It is found that the settlement of M. coruscus was promoted by the biofilms which are formed when the salinity is 13 or 23. Furthermore the adhesive rate reached maximum value 72%when the salinity is 13 or 23. It is indicated that the inducing activity of biofilm was positively correlated with salinity, biofilm age, dry weight and bacterial and diatom densities, but uncorrelated with chlorophyll a concentration. The dry weight, bacterial densities and diatom densities in biofilm increased with biofilm age, but chlorophylla was uncorrelated with biofilm age. Overall, this investigation indicates that bacterial structure may play an important role in the ad-hesion of the plantigrade M. coruscus, which can be induced by low salinity formed biofilms.