海洋科学
海洋科學
해양과학
MARINE SCIENCES
2013年
8期
55-60
,共6页
陈志%高如承%胡青%李鹏%赖素兰
陳誌%高如承%鬍青%李鵬%賴素蘭
진지%고여승%호청%리붕%뢰소란
响应面法%波纹巴非蛤(Paphia undulata)幼贝%生长率
響應麵法%波紋巴非蛤(Paphia undulata)幼貝%生長率
향응면법%파문파비합(Paphia undulata)유패%생장솔
response surface methodolody%Paphia undulata larvae%growth rate
为查明波纹巴非蛤(Paphia undulata)幼贝最适生长条件,采用响应面法研究了温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响。利用Box-Behnken中心组合进行3因素3水平的实验设计(3因素为温度(A),盐度(B),密度(C);3水平为-1,0,1),以幼贝生长率为响应值,以A(26℃,28℃,30℃)、B(28,30,32)和C(0.56,1.4,2.24个/cm2)为影响因子。实验结果表明:(1)盐度对幼贝生长影响最显著(P<0.05),3种生态因子对幼贝生长率影响程度大小顺序是:B>A>C;(2)通过SAS软件分析,得出波纹巴非蛤幼贝最适生长环境组合是温度为26.93℃、盐度为31.39、密度为1.12个/cm2,理论最佳生长率为41.47%。为验证响应面法预测结果的准确性,以预测结果为实验条件,相同实验时间后,得到的生长率为42.30%,结果与理论预测值相近,响应面方法可靠。
為查明波紋巴非蛤(Paphia undulata)幼貝最適生長條件,採用響應麵法研究瞭溫度、鹽度和密度交互作用對波紋巴非蛤幼貝生長的影響。利用Box-Behnken中心組閤進行3因素3水平的實驗設計(3因素為溫度(A),鹽度(B),密度(C);3水平為-1,0,1),以幼貝生長率為響應值,以A(26℃,28℃,30℃)、B(28,30,32)和C(0.56,1.4,2.24箇/cm2)為影響因子。實驗結果錶明:(1)鹽度對幼貝生長影響最顯著(P<0.05),3種生態因子對幼貝生長率影響程度大小順序是:B>A>C;(2)通過SAS軟件分析,得齣波紋巴非蛤幼貝最適生長環境組閤是溫度為26.93℃、鹽度為31.39、密度為1.12箇/cm2,理論最佳生長率為41.47%。為驗證響應麵法預測結果的準確性,以預測結果為實驗條件,相同實驗時間後,得到的生長率為42.30%,結果與理論預測值相近,響應麵方法可靠。
위사명파문파비합(Paphia undulata)유패최괄생장조건,채용향응면법연구료온도、염도화밀도교호작용대파문파비합유패생장적영향。이용Box-Behnken중심조합진행3인소3수평적실험설계(3인소위온도(A),염도(B),밀도(C);3수평위-1,0,1),이유패생장솔위향응치,이A(26℃,28℃,30℃)、B(28,30,32)화C(0.56,1.4,2.24개/cm2)위영향인자。실험결과표명:(1)염도대유패생장영향최현저(P<0.05),3충생태인자대유패생장솔영향정도대소순서시:B>A>C;(2)통과SAS연건분석,득출파문파비합유패최괄생장배경조합시온도위26.93℃、염도위31.39、밀도위1.12개/cm2,이론최가생장솔위41.47%。위험증향응면법예측결과적준학성,이예측결과위실험조건,상동실험시간후,득도적생장솔위42.30%,결과여이론예측치상근,향응면방법가고。
In order to explore the optimum growth conditions for Paphia undulata youth, response surface method-ology was used to study the effect of temperature, salinity and density on growth of Paphia undulata youth. The experiment was designed according to Box-Behnken central composite design in three factors and three levels, and temperature (26℃, 28℃, 30℃), salinity (28, 30, 32), density (0.56,1.4,2.24ind/cm2) were selected as affecting fac-tors. The results showed that the effect of salinity on the growth rate was the most significant (P <0.05), and the order of three ecological factors effects on the youth growth was salinity (B) > temperature (A) > density (C). By means of SAS software, the optimum growth environment condition of Paphia undulata youth was confirmed as follows: temperature 26.93℃, salinity 31.39 and density 1.12ind/cm2, and theoretical optimal growth rate was 41.47%. After experimental verification, the growth rate was 42.30%. To verify the accuracy of the results predicted by RSM, the real growth rate was 42.30%under the theoretical condition, suggesting RSM was reliable.