CAJ | 학술논문

利用6个微卫星位点研究了9个马氏珠母贝(Pinctada fucata)养殖家系的遗传结构,为后续育种工作提供了指导。从60对引物中筛选出6对能够稳定扩增且多态性较好的引物。6个微卫星位点在9个家系中共检测到17个等位基因,每个位点等位基因数(A)为2~4个,平均2.833个。6个位点在9个家系上的有效等位基因数(Ne)平均值为2.030~2.632,平均杂合度观测值(Ho)为0.4306~0.6354,平均杂合度期望值(He)为0.4380~0.5962。家系间遗传分化系数(FST)为0.0749。采用NJ法进行聚类分析显示,9个家系分为明显的两支,根据遗传距离计算结果, F7和F9家系之间的遗传距离最近, F11与F12家系遗传距离最远。结果表明,9个家系的遗传多样性和遗传分化处于中等水平,可以考虑F11与F12家系杂交,以期获得较好的杂种优势, F11自交培育出遗传稳定的优良家系。
이용6개미위성위점연구료9개마씨주모패(Pinctada fucata)양식가계적유전결구,위후속육충공작제공료지도。종60대인물중사선출6대능구은정확증차다태성교호적인물。6개미위성위점재9개가계중공검측도17개등위기인,매개위점등위기인수(A)위2~4개,평균2.833개。6개위점재9개가계상적유효등위기인수(Ne)평균치위2.030~2.632,평균잡합도관측치(Ho)위0.4306~0.6354,평균잡합도기망치(He)위0.4380~0.5962。가계간유전분화계수(FST)위0.0749。채용NJ법진행취류분석현시,9개가계분위명현적량지,근거유전거리계산결과, F7화F9가계지간적유전거리최근, F11여F12가계유전거리최원。결과표명,9개가계적유전다양성화유전분화처우중등수평,가이고필F11여F12가계잡교,이기획득교호적잡충우세, F11자교배육출유전은정적우량가계。
To further study the breeding strategy for Pinctada fucata, genetic structures of 9 families was investi-gated by using 6 microsatellite loci. 6 pairs of amplified and polymorphic primers are screened out from 60 pairs., and 17 alleles were discovered among 6 microsatellite loci. The number of alleles on each loci is 2 to 4 and the av-erage is about 2.833. The effective allele number ranged from 2.030 to 2.632. The mean observed value and mean expected value of heterozygosis were 0.4306 to 0.6354 and 0.4380 to 0.5962, respectively. Genetic differentiation coefficient (FST) reached 0.0749, demonstrating these 9 families had a moderately differentiation. 9 fimilies were divided into two clusters after clustering analysis by using NJ method. According to the calculation, the genetic distance between F7 and F9 was the nearest, the furthest one was between F11 and F12. It is suggested that F11 should be given prior consideration so as to gain stable genetic family by using selfing and to gain heterosis by hy-bridizing with F12.