CAJ | 학술논문

采用实验生态学的方法,通过染毒和清除,研究了不同浓度(0.05、0.5、5和10μg/L)苯并芘B[a]P胁迫15 d和释放15 d后,泥蚶(Tegillarca granosa)消化盲囊和鳃丝乙氧基异吩唑脱乙基酶(EROD)、谷胱甘肽转移酶(GST)活力和脂质过氧化物(MDA)含量的变化。结果表明：在胁迫阶段0.5、5和10μg/L, B[a]P处理组对泥蚶消化盲囊和鳃丝EROD、GST酶活力和MDA含量显著影响(P＜0.05), EROD、GST酶活力分别被诱导和抑制,第5 d趋于稳定, MDA含量随时间呈上升趋势,在第10 d 基本达到峰值并趋于稳定。在清除阶段, EROD活力和MDA含量逐渐下降, GST活力逐渐升高,并在5～10 d恢复到对照组水平。本研究中, EROD和GST活力的变化能够反映机体解毒代谢的能力, MDA含量的变化反映了机体氧化损伤的程度,表现出了一定的剂量和时间效应。
채용실험생태학적방법,통과염독화청제,연구료불동농도(0.05、0.5、5화10μg/L)분병비B[a]P협박15 d화석방15 d후,니감(Tegillarca granosa)소화맹낭화새사을양기이분서탈을기매(EROD)、곡광감태전이매(GST)활력화지질과양화물(MDA)함량적변화。결과표명：재협박계단0.5、5화10μg/L, B[a]P처리조대니감소화맹낭화새사EROD、GST매활력화MDA함량현저영향(P＜0.05), EROD、GST매활력분별피유도화억제,제5 d추우은정, MDA함량수시간정상승추세,재제10 d 기본체도봉치병추우은정。재청제계단, EROD활력화MDA함량축점하강, GST활력축점승고,병재5～10 d회복도대조조수평。본연구중, EROD화GST활력적변화능구반영궤체해독대사적능력, MDA함량적변화반영료궤체양화손상적정도,표현출료일정적제량화시간효응。
The effects of different concentrations of B[a]P (0.05、0.5、5 and 10μg/L) on EROD, GST and MDA in digestive gland and gills of Tegillarca granosa were studied during 15 days’ exposure to B[a]P. After B[a]P expo-sure, Tegillarca granosa were transferred to fresh seawater to be cultivated for 15 days. The results indicated that the activity of EROD, GST and MDA content were significantly effected in the 0.5, 5 and 10μg/L groups (P<0.05). EROD activity was induced while GST activity was inhibited until the fifth day. MDA content was rising with the treating time and arrived the peak, in the tenth day. All parameters were returned to the control level after 5-10 days’ clearance phase in the fresh seawater. According to our studies, the changes of EROD and GST activity could reflect the body's detoxification metabolism ability. The MDA content showed dose-effect and time-effect relationships, which indicated B[a]P exerted toxic effects on Tegillarca granosa by way of oxidative damage.