CAJ | 학술논문

以皱纹盘鲍(Haliotis discus hannai Ino)中国群体与日本群体的杂交(WJ)子1代为母本，在汕头养殖并选育了3代的“S”群体为父本，建立三元杂交组合(WJ-S)并尝试了海区围堰全生态模式养殖。以同期繁育的皱纹盘鲍中国群体与日本群体的杂交子1代(WJ-F1)为对照，比较 WJ-S 与对照组在生长速度和存活率等方面的差异。结果表明，在苗种和养成阶段， WJ-S 的度夏存活率均显著高于同期对照；从围口壳幼体到养成阶段， WJ-S组的平均壳长大于同期对照，养成阶段WJ-S组的全湿重显著高于对照，表明皱纹盘鲍三元杂交子代的生长速度显著高于对照；从苗种到养成阶段的3次分选数据表明， WJ-S的大规格子代比例均高于对照，小规格苗种比率则低于对照，表明三元杂交鲍苗种的生长速度优于对照；在青岛崂山海区全生态养殖18个月，三元杂交组已达商品规格。上述结果表明，在二元杂交的基础上加入第三方亲本 S 制备的三元杂交皱纹盘鲍，在生长速度、夏季高水温期的存活率等方面都较皱纹盘鲍“中-日”杂交子代显著提高。
이추문반포(Haliotis discus hannai Ino)중국군체여일본군체적잡교(WJ)자1대위모본，재산두양식병선육료3대적“S”군체위부본，건립삼원잡교조합(WJ-S)병상시료해구위언전생태모식양식。이동기번육적추문반포중국군체여일본군체적잡교자1대(WJ-F1)위대조，비교 WJ-S 여대조조재생장속도화존활솔등방면적차이。결과표명，재묘충화양성계단， WJ-S 적도하존활솔균현저고우동기대조；종위구각유체도양성계단， WJ-S조적평균각장대우동기대조，양성계단WJ-S조적전습중현저고우대조，표명추문반포삼원잡교자대적생장속도현저고우대조；종묘충도양성계단적3차분선수거표명， WJ-S적대규격자대비례균고우대조，소규격묘충비솔칙저우대조，표명삼원잡교포묘충적생장속도우우대조；재청도로산해구전생태양식18개월，삼원잡교조이체상품규격。상술결과표명，재이원잡교적기출상가입제삼방친본 S 제비적삼원잡교추문반포，재생장속도、하계고수온기적존활솔등방면도교추문반포“중-일”잡교자대현저제고。
We conducted a three-way crossing in Pacific abalone (Haliotis discus hannai Ino) with the WJ (♀) cohort, which was a hybrid between the wild populations from China (♀, W) and that from Japan (♂, J), and the S (♂) cohort which was selected for high growth rate in Shantou for three generations. The over-summering growth rate and survival rate were compared between the derived three-way cross (WJ-S) and the F1 generation of WJ (WJ-F1). The results showed that the average growth rate of the WJ-S cohort was significantly higher than that of WJ-F1 (P<0.05), which was also seen at commercial production scale. As a result, the proportion of large size adult abalones (>6 cm in shell length) in the WJ-S cohort was about 2 times more than that of WJ-F1. WJ-S also out per-formed the WJ-F1 in survival rate during over summering. The survival rate of the WJ-S cohort was 64.02%higher than that of WJ-F1 cohort during over-summering. In short, WJ-S exhibited higher growth and survival rate than WJ-F1, suggesting that three-way crossing may be applicable in aquaculture of Pacific abalone.