CAJ | 학술논문

本研究应用 AFLP 分子标记技术,对紫扇贝(Argopecten purpuratu, PP)、海湾扇贝(Argopecten irradians, II)、正交子一代(紫扇贝♀×海湾扇贝♂, PI)及反交子一代(海湾扇贝♀×紫扇贝♂, IP)群体的遗传结构进行了分析和比较。用5对AFLP引物扩增得到433个位点,其中多态位点为88.45%；正反交子代中的扩增位点大部分(97.23%)来源于紫扇贝和海湾扇贝；扩增中出现了非孟德尔遗传位点,如紫扇贝和海湾扇贝的部分扩增位点在杂交子代中丢失,正反交子代中出现12个紫扇贝和海湾扇贝中没有的扩增位点；通过多态位点比率、Nei’ s基因多样性、香农式多样性指数、遗传距离和AMOVA分析发现,正反杂交子代之间的遗传分化最小,与紫扇贝和海湾扇贝的遗传分化均显著。
본연구응용 AFLP 분자표기기술,대자선패(Argopecten purpuratu, PP)、해만선패(Argopecten irradians, II)、정교자일대(자선패♀×해만선패♂, PI)급반교자일대(해만선패♀×자선패♂, IP)군체적유전결구진행료분석화비교。용5대AFLP인물확증득도433개위점,기중다태위점위88.45%；정반교자대중적확증위점대부분(97.23%)래원우자선패화해만선패；확증중출현료비맹덕이유전위점,여자선패화해만선패적부분확증위점재잡교자대중주실,정반교자대중출현12개자선패화해만선패중몰유적확증위점；통과다태위점비솔、Nei’ s기인다양성、향농식다양성지수、유전거리화AMOVA분석발현,정반잡교자대지간적유전분화최소,여자선패화해만선패적유전분화균현저。
The AFLP molecular marker technique was used to analyze the genetic structure of Argopecten purpu-ratus (PP), Argopecten. irradians (II) and their hybrid offspring (A. purpuratus♀×A. irradians♂, PI;A. irradians♀×A. purpuratus♂, IP) in order to reveal the genetic differences and relationships between the parents and their offspring. Five AFLP primers were used to amplify the genome DNA, and 433 loci were yielded, the polymorphic loci of which accoutered for 88.45%. The majority of the amplified loci (97.23%) of the hybrid offspring were in-herited from both the parents, indicating that the hybrids were real heterogenous. Loci which not follow the Mende-lian Law were found in the analysis that some of the loci of the A. purpuratus and A. irradians were lost and 12 new loci were amplified in the hybrid offspring. It was revealed from the analysis of the percentage of polymorphic loci, Nei’s gene diversity, Shannon’s diversity index, genetic distance and AMOVA that the genetic differentiation be-tween the positisve and negative hybrid offspring was smaller than that between the offspring with their parents.