浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
9期
703-705
,共3页
大鼠%脓毒症%肾脏细胞%凋亡%Bcl-2%Bax%参麦注射液
大鼠%膿毒癥%腎髒細胞%凋亡%Bcl-2%Bax%參麥註射液
대서%농독증%신장세포%조망%Bcl-2%Bax%삼맥주사액
rats%sepsis renal cells%apoptosis%Bcl-2%Bax%Shenmai injection
目的：观察参麦注射液对脓毒症模型大鼠肾脏细胞凋亡及其Bcl-2和Bax蛋白表达的影响。方法：45只SD大鼠随机分为假手术组、脓毒症模型组和参麦组，每组15只。采用盲肠结扎穿刺法（CLP）造模。脓毒症模型组术前1h腹腔内注射生理盐水10mL/kg，假手术组除不结扎和穿刺盲肠外，其余操作同脓毒症模型组。参麦组术前1h腹腔内注射参麦注射液10mL/kg。术后24h取大鼠肾脏组织，采用TUNEL法检测并计算肾脏细胞凋亡指数（AI），免疫组化检测Bcl-2和Bax蛋白表达。结果：三组大鼠体质量差异无统计学意义（P跃0.05），三组大鼠均可见肾脏细胞凋亡,细胞凋亡多发生于肾小球细胞。脓毒症组和参麦组肾脏细胞AI均高于假手术组（P<0.01），参麦组肾脏细胞AI虽低于脓毒症组，但差异无统计学意义（P跃0.05）；与假手术组比较，脓毒症组和参麦组肾脏细胞Bcl-2表达下降（P<0.01，P<0.05），Bax表达上调（P<0.01），Bcl-2/Bax比值减小（P<0.01）。与脓毒症组比较，参麦组Bcl-2明显上调（P<0.01），Bax表达下调（P<0.05），Bcl-2/Bax比值增大（P<0.01）。结论：参麦注射液能上调脓毒症模型大鼠肾脏细胞Bcl-2蛋白表达，下调Bax蛋白表达，减少脓毒症大鼠肾脏细胞凋亡。
目的：觀察參麥註射液對膿毒癥模型大鼠腎髒細胞凋亡及其Bcl-2和Bax蛋白錶達的影響。方法：45隻SD大鼠隨機分為假手術組、膿毒癥模型組和參麥組，每組15隻。採用盲腸結扎穿刺法（CLP）造模。膿毒癥模型組術前1h腹腔內註射生理鹽水10mL/kg，假手術組除不結扎和穿刺盲腸外，其餘操作同膿毒癥模型組。參麥組術前1h腹腔內註射參麥註射液10mL/kg。術後24h取大鼠腎髒組織，採用TUNEL法檢測併計算腎髒細胞凋亡指數（AI），免疫組化檢測Bcl-2和Bax蛋白錶達。結果：三組大鼠體質量差異無統計學意義（P躍0.05），三組大鼠均可見腎髒細胞凋亡,細胞凋亡多髮生于腎小毬細胞。膿毒癥組和參麥組腎髒細胞AI均高于假手術組（P<0.01），參麥組腎髒細胞AI雖低于膿毒癥組，但差異無統計學意義（P躍0.05）；與假手術組比較，膿毒癥組和參麥組腎髒細胞Bcl-2錶達下降（P<0.01，P<0.05），Bax錶達上調（P<0.01），Bcl-2/Bax比值減小（P<0.01）。與膿毒癥組比較，參麥組Bcl-2明顯上調（P<0.01），Bax錶達下調（P<0.05），Bcl-2/Bax比值增大（P<0.01）。結論：參麥註射液能上調膿毒癥模型大鼠腎髒細胞Bcl-2蛋白錶達，下調Bax蛋白錶達，減少膿毒癥大鼠腎髒細胞凋亡。
목적：관찰삼맥주사액대농독증모형대서신장세포조망급기Bcl-2화Bax단백표체적영향。방법：45지SD대서수궤분위가수술조、농독증모형조화삼맥조，매조15지。채용맹장결찰천자법（CLP）조모。농독증모형조술전1h복강내주사생리염수10mL/kg，가수술조제불결찰화천자맹장외，기여조작동농독증모형조。삼맥조술전1h복강내주사삼맥주사액10mL/kg。술후24h취대서신장조직，채용TUNEL법검측병계산신장세포조망지수（AI），면역조화검측Bcl-2화Bax단백표체。결과：삼조대서체질량차이무통계학의의（P약0.05），삼조대서균가견신장세포조망,세포조망다발생우신소구세포。농독증조화삼맥조신장세포AI균고우가수술조（P<0.01），삼맥조신장세포AI수저우농독증조，단차이무통계학의의（P약0.05）；여가수술조비교，농독증조화삼맥조신장세포Bcl-2표체하강（P<0.01，P<0.05），Bax표체상조（P<0.01），Bcl-2/Bax비치감소（P<0.01）。여농독증조비교，삼맥조Bcl-2명현상조（P<0.01），Bax표체하조（P<0.05），Bcl-2/Bax비치증대（P<0.01）。결론：삼맥주사액능상조농독증모형대서신장세포Bcl-2단백표체，하조Bax단백표체，감소농독증대서신장세포조망。
Objective:To investigate the effects of Shenmai injection on renal apoptosis and Bcl-2/Bax protein expres-sion in septic rats. Methods:Forty-five male SD rats were randomly divided into sham operation group,sepsis group,and drug group,15 rats in each group. The sepsis model was made by cecal ligation and puncture(CLP) modeling. In the sepsis group the rats were injected with 10 mL/kg saline into their peritoneal cavities 1 h before CLP. The rats in the sham operation group were treated the same way except no ligation and puncture of the ce-cum. In the drug group the rats were injected with 10 mL/kg Shenmai injection fluid into their peritoneal cavities 1 h before CLP. All rats were given subcutaneous injection of 50 mL/kg Ringer's solution for anti-shock immedi-ately after operation. In 24 h after operation, renal specimens of the rats were collected. The renal apoptosis was detected by using TUNEL method. The Bcl-2 and Bax protein expression was determined by using immunohisto-chemistry. Results:Six rats were died in the sepsis group and 4 rats in the drug group. No significant difference in body weight was noted among the three groups (P>0.05). The renal apoptosis index in the sepsis and drug groups were higher than that in the sham operation group (P<0.01),while that in the drug group was lower than that in the sham operation group without a significant difference (P=0.084). Compared with the sham operation group,the Bcl-2 protein decreased(P<0.01,P<0.05) and the Bax protein increased (P<0.01),and the Bcl-2/Bax ratio reduced (P<0.01) in the sepsis and drug groups. Compared with the sepsis group,the Bcl-2 protein increased(P<0.01) and the Bax protein decreased (P<0.05),and the Bcl-2/Bax ratio increased (P<0.01) in the drug group. Conclusion:Shenmai injection can upregulate the Bcl-2 protein and downregulate the Bax protein,resulting in prevention from apoptosis in rats.
