CAJ | 학술논문

文昌鱼作为研究脊椎动物起源和进化的模式生物，由于缺乏自由循环的血细胞，关于其清除体内病原体的机制，迄今尚无报道。采用体内细菌清除试验、体外杀菌试验、特异性抗体抑制试验、抑制补体途径试验及Western blot方法，研究了文昌鱼体内细菌清除机制及动力学。试验结果表明，文昌鱼能有效清除入侵的异源微生物，体液对大肠杆菌具有较强的杀灭活性。补体和溶菌酶是参与清除入侵大肠杆菌的主要因子，通过替代途径激活的补体较溶菌酶作用更强，这是关于文昌鱼清除体内细菌机制及其相关因子的首次报道。
문창어작위연구척추동물기원화진화적모식생물，유우결핍자유순배적혈세포，관우기청제체내병원체적궤제，흘금상무보도。채용체내세균청제시험、체외살균시험、특이성항체억제시험、억제보체도경시험급Western blot방법，연구료문창어체내세균청제궤제급동역학。시험결과표명，문창어능유효청제입침적이원미생물，체액대대장간균구유교강적살멸활성。보체화용균매시삼여청제입침대장간균적주요인자，통과체대도경격활적보체교용균매작용경강，저시관우문창어청제체내세균궤제급기상관인자적수차보도。
It remains unknown to date about the mechanism that pathogens are eliminated from amphioxus (Branchiostoma belcheri) lacking free circulating blood cells even though amphioxus is widely used as the model organism for evolution and development research .The bacterium clearance dynamics in amphioxus were studied in vivo and in vitro by lysis of Escherichia coli ,inhibition of lytic activity with the specific antibodies ,and western blot .It was found that the amphioxus was capable of efficiently eliminating the invading bacterium Escherichia coli and that the humoral fluids can readily lyse the bacterium in v itro . Both complement and lysozyme were shown to be involved in the elimination in vivo of the invading bacteria in amphioxus and to act in concert against the invading bacteria , the complement playing a stronger role than the lysozyme doing . Western blot showed that both C3α and lysozyme levels were increased with the challenge with E .coli .This is the first report addressing the factors responsible for the clearance in v ivo of invading bacteria in amphioxus .