CAJ | 학술논문

在基因分离与克隆中，构建各种cDNA文库是近些年在发现新基因及研究基因功能常用的基础工具之一。其中，抑制性消减杂交技术是一种高效分离差异表达基因的方法，在分离筛选细菌活的非可培养状态与正常菌株差异基因中，应用这种研究方法是研究细菌VBNC状态株毒力、致病性和耐药性及菌株遗传分化关系的重要途径。本文旨在介绍SSH构建消减cDNA文库在原核生物中的应用及对其在细菌VBNC状态新基因筛选中的应用前景进行综合论述，并对其进一步研究提出了展望。
재기인분리여극륭중，구건각충cDNA문고시근사년재발현신기인급연구기인공능상용적기출공구지일。기중，억제성소감잡교기술시일충고효분리차이표체기인적방법，재분리사선세균활적비가배양상태여정상균주차이기인중，응용저충연구방법시연구세균VBNC상태주독력、치병성화내약성급균주유전분화관계적중요도경。본문지재개소SSH구건소감cDNA문고재원핵생물중적응용급대기재세균VBNC상태신기인사선중적응용전경진행종합논술，병대기진일보연구제출료전망。
cDNA library constructions are basic tools of detecting new gene and re-searching gene function in gene separation and cloning in recent years. Suppression Subtractive Hybridization is one of the most efficient method in isolating differen-tially expressed genes between viable but non-culturable state bacteria and normal strains, SSH is the important way in studying virulence, pathogenicity, drug resis-tance and genetic differentiation relations of strains in VBNC bacteria. The objec-tive of this paper was to discuss the construction strategies of cDNA by SSH in pro-karyotic organism and its application to isolate differentially expressed genes in VBNC bacteria, and it put forward the outlook for further research.