CAJ | 학술논문

本研究建立了饲料中玉米赤霉醇类药物的UPLC-MS/MS分析方法。饲料样品经乙腈水溶液提取，离心后提取液经多功能净化柱净化，氮气吹干，用乙腈+乙酸水溶液（50：50，V/V）溶解， UPLC-MS/MS分析。结果显示，6种玉米赤霉醇类药物在5.0～50.0 g/kg浓度范围内线性关系良好， r≥0.999。以单一饲料、浓缩饲料、配合饲料和预混料等为添加基质，其回收率在65.8%～104.6%，检出限为5.0 g/kg。本方法灵敏度高、专属性好、操作简单、结果可靠，可满足饲料中玉米赤霉醇类药物的分析检测。
본연구건립료사료중옥미적매순류약물적UPLC-MS/MS분석방법。사료양품경을정수용액제취，리심후제취액경다공능정화주정화，담기취간，용을정+을산수용액（50：50，V/V）용해， UPLC-MS/MS분석。결과현시，6충옥미적매순류약물재5.0～50.0 g/kg농도범위내선성관계량호， r≥0.999。이단일사료、농축사료、배합사료화예혼료등위첨가기질，기회수솔재65.8%～104.6%，검출한위5.0 g/kg。본방법령민도고、전속성호、조작간단、결과가고，가만족사료중옥미적매순류약물적분석검측。
A method of Ultra Performance Liquid Chromatography-Tandem mass spectrom-etry (UPLC-MS/MS) for determination of Zearalanol and Analogues in feeds was devel-oped. The drugs was extracted with acetonitrile / water from analyte, then cleaned up with multifunction cartridges. The eluent was evaporated by nitrogen blow, dis-solved in acetonitrile / water and then assayed by UPLC-MS/MS. The limits of detec-tion were 5.0 μg/kg for Zearalanol and Analogues and the average recoveryies were 65.8%~104.6%. The real sample tests showed this method can be used for the sensi-tive and accurate determination of Zearalanol and Analogues in feeds.