CAJ | 학술논문

目的探讨呼吸道合胞病毒（RSV）毛细支气管炎（毛支）患儿急性期外周血辅助性T细胞17（Th17）和白介素17（IL-17）水平的变化及作用。方法选取RSV毛支急性期患儿（n=28，其中轻度组16例、重度组12例）为毛支组，以哮喘急性发作儿童（n=12）为哮喘组，近期无感染的小儿外科术前患儿（n=10）为对照组。以流式细胞术检测外周血Th17表达，ELISA方法检测外周血IL-17；分析RSV毛支患儿急性期外周血Th17细胞和IL-17表达与病情严重程度的关系。结果毛支重度组外周血Th17细胞比例和IL-17水平分别高于轻度组和对照组，差异均有统计学意义(P＜0.05)；但与哮喘组比较，差异无统计学意义(P＞0.05)。结论 RSV毛细支气管炎患儿急性期外周血Th17细胞和IL-17表达水平增加，Th17细胞和IL-17可能参与RSV毛细支气管炎的发病机制。
목적탐토호흡도합포병독（RSV）모세지기관염（모지）환인급성기외주혈보조성T세포17（Th17）화백개소17（IL-17）수평적변화급작용。방법선취RSV모지급성기환인（n=28，기중경도조16례、중도조12례）위모지조，이효천급성발작인동（n=12）위효천조，근기무감염적소인외과술전환인（n=10）위대조조。이류식세포술검측외주혈Th17표체，ELISA방법검측외주혈IL-17；분석RSV모지환인급성기외주혈Th17세포화IL-17표체여병정엄중정도적관계。결과모지중도조외주혈Th17세포비례화IL-17수평분별고우경도조화대조조，차이균유통계학의의(P＜0.05)；단여효천조비교，차이무통계학의의(P＞0.05)。결론 RSV모세지기관염환인급성기외주혈Th17세포화IL-17표체수평증가，Th17세포화IL-17가능삼여RSV모세지기관염적발병궤제。
Objectives To study the roles of Th17 cells in peripheral blood and the serum level of IL-17 in children with acute respiratory syncytial virus (RSV) bronchiolitis. Methods A total of 28 children with RSV bronchiolitis were selected as bronchiolitis group, among whom twelve cases were assigned into severe group and sixteen cases were assigned into mild group according to the severity of illness. Twelve cases with acute asthma were selected as asthma group. Ten children without recent infection waiting for surgery were chosen as controls. The percentage of Th17 cells in peripheral blood was measured by lfow cytometry. The serum level of IL-17 was detected by ELISA. The association of severity of illness with the percentage of Th17 cells and the level of IL-17 was studied in children with acute RSV bronchiolitis. Results The serum level of IL-17 and the percentage of Th17 cells were signiifcantly higher in the severe bronchiolitis group than those in the control group and mild bronchiolitis group (P<0.05). However, there was no signiifcant difference between the severe bronchiolitis group and the asthma group (P>0.05). Conclusions The percentage of Th17 cells and serum level of IL-17 are increased in children with acute RSV bronchiolitis, and may be involved in the pathogenesis of RSV bronchiolitis. (J Clin Pediatr,2013,31(9):850-853)