CAJ | 학술논문

目的对hsa-miR-1908进行系统的生物信息学分析，预测其可能参与的生物学过程及信号通路，为深入研究其在人脂肪细胞分化、肥胖发生等过程中的功能与机制奠定基础。方法应用miRBase获取hsa-miR-1908的序列，并分析其特征；应用miRanda预测hsa-miR-1908的靶基因，并取预测结果及基因芯片结果的交集，进一步进行基因功能注释（Gene ontology）和生物通路富集分析（Pathway enrichment)。结果 hsa-miR-1908在各物种之间有一定保守性，其靶基因功能富集于Wnt受体信号的调控、细胞周期、细胞凋亡等生物学过程；其靶基因信号通路富集于促性腺激素释放激素信号通路、MAPK信号通路、胰岛素信号通路、细胞周期等信号转导通路及胰腺癌等疾病通路中。结论 hsa-miR-1908预测的靶基因集合富集于多个信号通路及生物学过程，与肥胖密切相关，为后续has-miR-1908在人脂肪细胞中生物学功能研究奠定基础。
목적대hsa-miR-1908진행계통적생물신식학분석，예측기가능삼여적생물학과정급신호통로，위심입연구기재인지방세포분화、비반발생등과정중적공능여궤제전정기출。방법응용miRBase획취hsa-miR-1908적서렬，병분석기특정；응용miRanda예측hsa-miR-1908적파기인，병취예측결과급기인심편결과적교집，진일보진행기인공능주석（Gene ontology）화생물통로부집분석（Pathway enrichment)。결과 hsa-miR-1908재각물충지간유일정보수성，기파기인공능부집우Wnt수체신호적조공、세포주기、세포조망등생물학과정；기파기인신호통로부집우촉성선격소석방격소신호통로、MAPK신호통로、이도소신호통로、세포주기등신호전도통로급이선암등질병통로중。결론 hsa-miR-1908예측적파기인집합부집우다개신호통로급생물학과정，여비반밀절상관，위후속has-miR-1908재인지방세포중생물학공능연구전정기출。
Objective To predict the biological process and signaling pathways in which hsa-miR-1908 might be in-volved by a series of bioinformatics analysis, so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-1908 biological function in human preadipocytes. Methods The sequence of hsa-miR-1908 was acquired from miR-Base database, and target genes of hsa-miR-1908 were predicted by miRanda, and then the intersection of the results and the results of gene-chip as gene set were further analyzed by gene ontology and pathway enrichment. Results The hsa-miR-1908 had some conserved property among different species. The functions of the target genes were enriched in Wnt receptor signal-ing pathway through beta-catenin, cell cycle, cell apeptosis and other biological processes. The GnRH signaling, MAPK sig-naling, insulin signaling, cell cycle signal transduction pathways and signaling pathway in pancreatic cancer were signiifcantly enriched. Conclusions The target genes set of hsa-miR-1908 were enriched in multiple biological process which are related with the obesity. This study provides guidance for the further study in human preadipocytes.