CAJ | 학술논문

在全基因组水平挖掘了26个苹果的CDPK基因，并通过基因结构和结构域组织的比较得到各成员之间的异同点，再通过蛋白质三级结构的分析，获得了苹果CDPK在重要的关键位点的突变，而这可能引起结构的自抑制和激活的重大差别。结合多个物种CDPK的挖掘，并与苹果CDPK各成员建立的系统发育树进行分析，得到了苹果26个CDPK的起源和21次扩增历史。借助于苹果CDPK表达的EST数据了解了苹果CDPK的初步表达情况，这些完善的苹果CDPK成员间复杂的进化历史对于开展后续验证成员间彼此的功能联系以及基因的利用奠定了基础。
재전기인조수평알굴료26개평과적CDPK기인，병통과기인결구화결구역조직적비교득도각성원지간적이동점，재통과단백질삼급결구적분석，획득료평과CDPK재중요적관건위점적돌변，이저가능인기결구적자억제화격활적중대차별。결합다개물충CDPK적알굴，병여평과CDPK각성원건립적계통발육수진행분석，득도료평과26개CDPK적기원화21차확증역사。차조우평과CDPK표체적EST수거료해료평과CDPK적초보표체정황，저사완선적평과CDPK성원간복잡적진화역사대우개전후속험증성원간피차적공능련계이급기인적이용전정료기출。
The 26 characterized Calcium -dependent Protein Kinase ( CDPK) genes were encoded from the genome of apple ( Malus domestica) .The similarities and differences among CDPK members were acquired by comparison of gene structure and do-main organization .Through the analysis of third -class structure of protein , the mutation of apple CDPK at key locus was obtained , which might cause major differences in auto -inhibition and activation of CDPK structure.The phylogenetic tree of the members of apple CDPK was analyzed , which got the origin of the 26 CDPKs and 21 amplifications .The preliminary expression of CDPK in ap-ple was understood by EST data .The complex evolutionary history of perfect apple CDPK members laid the foundation on carrying out the validation of functional contact and gene utilization .