CAJ | 학술논문

目的研究促红细胞生成素（EPO）对衰老大鼠脑组织中抗氧化酶活性的影响。方法将大鼠随机分为3组：正常对照组、衰老模型组及重组人促红细胞生成素（rhEPO）干预组，每组10只。行为学实验后处死大鼠，行尼氏染色在光镜下观察海马组织神经元内的尼氏体数量；同时检测脑组织中过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH-Px）的活性。结果①衰老组较对照组大鼠学习记忆能力下降（P<0.05），干预组较衰老组大鼠的学习记忆能力改善（P<0.05）；②光镜下见衰老组大鼠神经元内尼氏体数量较对照组明显减少，干预组较衰老组明显增多，但少于对照组；③衰老组大鼠脑组织中的CAT、GSH-Px活性均较对照组显著降低（P<0.05），而干预组的CAT、GSH-Px活性较衰老模型组显著升高（P<0.05）。结论 EPO可增强衰老大鼠脑组织中抗氧化酶的活性，提高其抗氧化能力从而起到抗衰老作用。
목적연구촉홍세포생성소（EPO）대쇠로대서뇌조직중항양화매활성적영향。방법장대서수궤분위3조：정상대조조、쇠로모형조급중조인촉홍세포생성소（rhEPO）간예조，매조10지。행위학실험후처사대서，행니씨염색재광경하관찰해마조직신경원내적니씨체수량；동시검측뇌조직중과양화경매（CAT）급곡광감태과양화물매（GSH-Px）적활성。결과①쇠로조교대조조대서학습기억능력하강（P<0.05），간예조교쇠로조대서적학습기억능력개선（P<0.05）；②광경하견쇠로조대서신경원내니씨체수량교대조조명현감소，간예조교쇠로조명현증다，단소우대조조；③쇠로조대서뇌조직중적CAT、GSH-Px활성균교대조조현저강저（P<0.05），이간예조적CAT、GSH-Px활성교쇠로모형조현저승고（P<0.05）。결론 EPO가증강쇠로대서뇌조직중항양화매적활성，제고기항양화능력종이기도항쇠로작용。
Objective To study the effect of erythropoietin (EPO) on the activities of antioxidant enzymes, namely catalase (CAT) and glutathione peroxidase (GSH-Px) in the brain tissues of aged rats. Methods Thirty SD rats were randomly divided into normal control, aging model, and recombinant human erthropoietin (rhEPO) treatment groups (n=10). Morris water maze was used to compare the behavioral indexes. The rats were then sacrificed to observe Nissl bodies in the hippocampal neurons with Nissl staining and test the activities of CAT and GSH-Px in the brain tissues. Results Compared with the control group, the aging rats showed significantly deteriorated learning and memory abilities (P<0.05), which were improved obviously by rhEPO treatment (P<0.05). The number of Nissl bodies in the neurons was reduced in the aging rats compared with that in the control group, and rhEPO treatment increased the number of Nissle bodies but failed to restore the control level. The aging rats also showed significantly lowered activities of CAT and GSH-Px in the brain tissue (P<0.05), which were increased significantly after rhEPO treatment (P<0.05). Conclusion EPO can enhance the activities of the antioxidant enzymes in the brain tissues of aged rats to increase the antioxidant capacity and produces an anti-aging effect.