CAJ | 학술논문

目的研究水通道蛋白基因4（AQP4）在子痫前期模型鼠脑组织中的表达变化。方法成年SD大鼠分为PE模型组（7只）通过尾静脉注射内毒素（1μg/kg）的方法建立，正常妊娠组（6只）和非孕组（12只）注射等量生理盐水。应用免疫组化和Western-blot检测各组大鼠海马区AQP4的表达水平。结果妊娠第19日PE模型组大鼠血压（135±9）mmHg明显高于正常妊娠组（116±8）mmHg和非孕组（112±6）mmHg（P<0.02）；PE模型组尿蛋白（3.8±0.5）水平明显高于正常妊娠组（2.6±0.6）和非孕组（2.3±0.4）（P<0.05）。免疫组化结果显示AQP4集中分布在脑内血管壁，Western-blot灰度分析提示子痫前期大鼠海马组织中AQP4蛋白的表达水平（41.7±4.2）明显高于正常妊娠组（26.3±5.3）和非孕组（9.2±2.1）（P<0.01）。结论 AQP4在子痫前期模型大鼠脑组织中表达上调，可能与子痫发病有关。
목적연구수통도단백기인4（AQP4）재자간전기모형서뇌조직중적표체변화。방법성년SD대서분위PE모형조（7지）통과미정맥주사내독소（1μg/kg）적방법건립，정상임신조（6지）화비잉조（12지）주사등량생리염수。응용면역조화화Western-blot검측각조대서해마구AQP4적표체수평。결과임신제19일PE모형조대서혈압（135±9）mmHg명현고우정상임신조（116±8）mmHg화비잉조（112±6）mmHg（P<0.02）；PE모형조뇨단백（3.8±0.5）수평명현고우정상임신조（2.6±0.6）화비잉조（2.3±0.4）（P<0.05）。면역조화결과현시AQP4집중분포재뇌내혈관벽，Western-blot회도분석제시자간전기대서해마조직중AQP4단백적표체수평（41.7±4.2）명현고우정상임신조（26.3±5.3）화비잉조（9.2±2.1）（P<0.01）。결론 AQP4재자간전기모형대서뇌조직중표체상조，가능여자간발병유관。
Objective To study the expression of aquaporin-4 (AQP4) protein in preeclampsia(PE) model rats. Methods Adult SD rats were divided into PE model group (n=7) induced by endotoxin, normal pregnancy group (n=6) and non-pregnancy group (n=12) treated with an equal volume of saline. AQP4 protein expression in the brain of the rats was detected with immunohistochemical staining and Western blotting. Results The blood pressure in PE model rats (135 ± 9 mmHg) was significantly higher than that in normal pregnancy rats (116 ± 8 mmHg) and non-pregnancy rats (112 ± 6 mmHg) (P<0.02). The rats in PE model group showed obvious proteinuria compared with the other two groups (3.8 ± 0.5 vs 2.6 ± 0.6 and 2.3 ± 0.4, P<0.05). Immunohistochemistry showed that AQP4 protein was localized primarily around the brain parenchymal blood vessels. Western analysis revealed a significantly elevated AQP4 protein expression in PE model group (39.6 ± 4.9) compared with that in normal pregnancy group (26.5 ± 4.3) and non-pregnancy group (9.7 ± 2.1) (P<0.01). Conclusion The up-regulation of AQP4 protein around the intraparenchymal blood vessels of the brain may play a role in the development of eclampsia.