CAJ | 학술논문

目的探讨miR-19a对溃疡性结肠炎的作用机制。方法通过生物信息学分析预测miR-19a可能的靶基因，通过免疫组化及Western blotting技术检测靶基因在溃疡性结肠炎小鼠中表达的变化，并进一步通过绿色荧光蛋白报告载体实验对靶基因进行鉴定。结果生物信息学分析预测miR-19a可能的靶基因是TNF-α，免疫组化及Western blotting显示溃疡性结肠炎小鼠肠道组织TNF-α表达增加，miR-19a可抑制TNF-α-3'UTR-WT报告基因活性，而变异型TNF-α-3'UTR-mut报告基因活性不能被抑制。结论miR-19a的靶基因为TNF-α，其结合位点为TNF-α3'UTR，miR-19a可能在肠道通过直接调控TNF-α而发挥作用。
목적탐토miR-19a대궤양성결장염적작용궤제。방법통과생물신식학분석예측miR-19a가능적파기인，통과면역조화급Western blotting기술검측파기인재궤양성결장염소서중표체적변화，병진일보통과록색형광단백보고재체실험대파기인진행감정。결과생물신식학분석예측miR-19a가능적파기인시TNF-α，면역조화급Western blotting현시궤양성결장염소서장도조직TNF-α표체증가，miR-19a가억제TNF-α-3'UTR-WT보고기인활성，이변이형TNF-α-3'UTR-mut보고기인활성불능피억제。결론miR-19a적파기인위TNF-α，기결합위점위TNF-α3'UTR，miR-19a가능재장도통과직접조공TNF-α이발휘작용。
Objective To study the role of miR-19a in ulcerative colitis (UC) in mice. Methods The target gene of miR-19a was predicted by bioinformatics analysis. The expression of the target protein in UC colon was detected by immunohistochemistry and Western blotting. The target gene was further identified by enhanced green fluorescent protein (EGFP) report vector system. Results The target gene of miR-19a was TNF-αas predicted by bioinformatics analysis. TNF-αexpression was highly expressed in the colonic tissue of UC mice. MiR-19a could inhibit the report gene activity of TNF-α-3'UTR-WT but no that of TNF-α-3'UTR-Mut. Conclusion The target gene of miR-19a is TNF-α, and the binding site is TNF-α3'UTR. The possible role of miR-19a in UC pathogenesis involves regulation of TNF-αexpression in the colon.