CAJ | 학술논문

目的探讨NOD样受体热蛋白结构域相关蛋白3（NLRP3）炎症小体在咪喹莫特诱导的小鼠银屑病样模型中的作用及芥菜籽对NLRP3炎症小体的影响。方法将30只BALB/c小鼠随机分为3组，正常对照组、模型组(给予正常饲料并用5%咪喹莫特诱导银屑病样炎症)和实验组（给予5%芥菜籽饲料并用5%咪喹莫特诱导银屑病样炎症）。RT-PCR方法检测皮损处NLRP3、ASC、caspase-1和caspase-11的mRNA表达，免疫组化方法检测ASC和caspase-1的表达和分布，ELISA方法检测血清IL-1β和IL-18的含量。结果与正常对照组相比，小鼠银屑病样模型皮损中NLRP3、ASC、caspase-1和caspase-11的表达水平增高，血清IL-1β和IL-18的含量增高，差异有统计学意义（P<0.05）；与模型组相比，芥菜籽抑制上述因子在实验组中的表达，差异有统计学意义（P<0.05）。结论 NLRP3炎症小体可能参与了银屑病样模型的形成，芥菜籽可能通过降低其表达抑制了IL-1β、IL-18引起的炎症反应而减轻银屑病损害。
목적탐토NOD양수체열단백결구역상관단백3（NLRP3）염증소체재미규막특유도적소서은설병양모형중적작용급개채자대NLRP3염증소체적영향。방법장30지BALB/c소서수궤분위3조，정상대조조、모형조(급여정상사료병용5%미규막특유도은설병양염증)화실험조（급여5%개채자사료병용5%미규막특유도은설병양염증）。RT-PCR방법검측피손처NLRP3、ASC、caspase-1화caspase-11적mRNA표체，면역조화방법검측ASC화caspase-1적표체화분포，ELISA방법검측혈청IL-1β화IL-18적함량。결과여정상대조조상비，소서은설병양모형피손중NLRP3、ASC、caspase-1화caspase-11적표체수평증고，혈청IL-1β화IL-18적함량증고，차이유통계학의의（P<0.05）；여모형조상비，개채자억제상술인자재실험조중적표체，차이유통계학의의（P<0.05）。결론 NLRP3염증소체가능삼여료은설병양모형적형성，개채자가능통과강저기표체억제료IL-1β、IL-18인기적염증반응이감경은설병손해。
Objective To investigate the role of NLRP3 inflammasome in imiquimod-induced psoriasis-like inflammation in mice and the therapeutic effects of mustard seed (Sinapis Alba Linn). Methods Thirty BALB/c mice were randomized equally into blank control group (fed with normal forage and treated with vehicle), model group (fed with normal forage and treated with 5% imiquimod cream), and experimental group (fed with 5% mustard seed forage and treated with 5% imiquimod cream). RT-PCR was used to detect the mRNA expression of NLRP3, ASC, caspase-1, and caspase-11. Immunohistochemistry was performed to determine the expression and distribution of ASC and caspase-1. ELISA was used to test the serum levels of interleukin-1β (IL-1β) and IL-18. Results Compared with the blank control group, the mice with imiquimod-induced psoriasis-like inflammation showed significantly increased NLRP3, ASC, caspase-1, and caspase-11 mRNA expressions, ASC and caspase-1 protein expressions , and serum levels of IL-1βand IL-18 (P<0.05). These changes were obviously attenuated by feeding the mice with mustard seed. Conclusion NLRP3 inflammasome is involved in imiquimod-induced psoriasis-like inflammation in mice, and mustard seed may suppress the inflammation induced by IL-1βand IL-18 through down-regulating the expression of NLRP3 inflammasome.