CAJ | 학술논문

本文建立了紫外分光光度计法准确测定马铃薯中的总多酚。对影响Folin-Ciocalteus法测定马铃薯中总多酚含量的主要因素进行了研究，确定了在1.0 mL样品提取液或标液中依次加入5.0 mL水、1.0 mL显色剂和3.0 mL 7.5％碳酸钠溶液，摇匀，显色2 h后在765 nm测定吸光度的最佳检测条件，并对马铃薯样品进行了测定，精密度为7.7％，回收率为100%~108%。本方法适用于常规实验室马铃薯总多酚的测定。
본문건립료자외분광광도계법준학측정마령서중적총다분。대영향Folin-Ciocalteus법측정마령서중총다분함량적주요인소진행료연구，학정료재1.0 mL양품제취액혹표액중의차가입5.0 mL수、1.0 mL현색제화3.0 mL 7.5％탄산납용액，요균，현색2 h후재765 nm측정흡광도적최가검측조건，병대마령서양품진행료측정，정밀도위7.7％，회수솔위100%~108%。본방법괄용우상규실험실마령서총다분적측정。
An improved UV spectrophotometry method was developed for analysis of total polyphenols in potato. Folin-Ciocalteus method for total polyphenols determination in potato was studied. After a series of optimization experiments, the best determination procedure of Folin-Ciocalteus method was put forward, that is, 5.0 mL water, 1.0 mL coloration reagent, and 3.0 mL 7.5%sodium carbonate solution were added orderly into 1.0 mL extraction of sample or standard gal ic acid, then blended, and measured at 765 nm after coloration for 2 h. Under this procedure, relative standard deviation of the method was 7.7%, and average recovery rate was 100%-108%.This method is applicable to conventional laboratory determination of total polyphenols in potato.