CAJ | 학술논문

以强休眠性玉米自交系08-641为材料,2个弱休眠性玉米自交系为对照,利用蛋白质双向电泳技术,对处于休眠状态下的新鲜收获种子和经过15 d后熟处理破除休眠的3个自交系种子进行了蛋白质差异表达分析。结果表明,在3次重复试验下共检测到9个与休眠相关的蛋白点,其中新增诱导表达蛋白质1个,缺失表达蛋白质2个,上调表达蛋白质5个,下调表达蛋白质1个。在9个蛋白质中,有5个蛋白点得到鉴定。包括3个globulin-1 S allele precursor、1个2-isopropylmalate synthase B和1个translationally-controlled tumor protein。种子休眠破除过程中的蛋白质的变化说明种子经历了一系列生理生化活动,深入研究这些蛋白质的生物学功能将有助于更清楚地认识玉米种子休眠问题。
이강휴면성옥미자교계08-641위재료,2개약휴면성옥미자교계위대조,이용단백질쌍향전영기술,대처우휴면상태하적신선수획충자화경과15 d후숙처리파제휴면적3개자교계충자진행료단백질차이표체분석。결과표명,재3차중복시험하공검측도9개여휴면상관적단백점,기중신증유도표체단백질1개,결실표체단백질2개,상조표체단백질5개,하조표체단백질1개。재9개단백질중,유5개단백점득도감정。포괄3개globulin-1 S allele precursor、1개2-isopropylmalate synthase B화1개translationally-controlled tumor protein。충자휴면파제과정중적단백질적변화설명충자경력료일계렬생리생화활동,심입연구저사단백질적생물학공능장유조우경청초지인식옥미충자휴면문제。
Because the special natural climate of overabundant rain and poor sunshine in the southwest, pre-harvest sprouting has seriously affected the quality and yield of maize, looking for a good material with resistance to pre-harvest sprouting is the urgent need for improvement of existing maize strains. The results of this study indicated that proteome of 08-641, 008, and 127 seeds significantly changed during post-ripening. Nine differentially expressed proteins related to dormancy in the seeds of maize inbred line 08-641 with 15 days afterripening process were analysed through 2-DE, including one newly induced expressed protein, two missing expressed proteins, five up-regulated expressed proteins, and one down-regulated expressed protein. Among the nine proteins, five proteins were identified by mass spectrometry, including three globulin-1 S allele precursor of storage proteins, one 2-isopropylmalate synthase B and one translationally-controlled tumor protein involved in regulating protein structure and cell function. The changes of proteins during dry ripening process of seeds indicated that the seeds undergo a series of physiological and biochemical activities befor they can germinate normally, and the deeply study on the biological function of these proteins will help us understand maize seed dormancy more clearly.