CAJ | 학술논문

采用籽粒离体培养的方法，研究花后高温对玉米强、弱势籽粒的影响。结果表明，高温处理加快了强、弱势籽粒前期的灌浆速率，但降低了中后期的灌浆速率，导致粒重降低，且对弱势粒影响尤为显著，高温处理强、弱势粒成熟期粒重分别比对照低5.8%、17.4%；高温显著降低了籽粒不同灌浆时期的淀粉合成相关酶活性，从而使淀粉含量降低，强势粒的淀粉含量降低幅度小于弱势粒；与对照相比，高温处理后强势籽粒中的3-吲哚乙酸(IAA)和玉米素核苷(ZR)含量显著下降，赤霉素(GA3)含量则无显著差异，而弱势粒IAA、ZR含量显著降低，但GA3含量增加，可能是导致弱势粒干重受损较大的原因。
채용자립리체배양적방법，연구화후고온대옥미강、약세자립적영향。결과표명，고온처리가쾌료강、약세자립전기적관장속솔，단강저료중후기적관장속솔，도치립중강저，차대약세립영향우위현저，고온처리강、약세립성숙기립중분별비대조저5.8%、17.4%；고온현저강저료자립불동관장시기적정분합성상관매활성，종이사정분함량강저，강세립적정분함량강저폭도소우약세립；여대조상비，고온처리후강세자립중적3-신타을산(IAA)화옥미소핵감(ZR)함량현저하강，적매소(GA3)함량칙무현저차이，이약세립IAA、ZR함량현저강저，단GA3함량증가，가능시도치약세립간중수손교대적원인。
The kernel culture in vitro was adopted to study the effect of high temperature after flowering on maize superior and inferior kernels. Results indicated that dry matter accumulation of grain decreased, due to the fact that grain development was accelerated at early grain-filling stage by high temperature, but declined at middle late grain filling stage. In mature stage, the dry weight of superior and inferior kernels under high temperature was reduced by 5.8%and 17.4%, respectively, so inferior kernels had a more reduction than superior kernels. The synthesis of starch was influenced by reducing the activity of enzymes related to starch synthesis significantly at different grain-filling stages under high temperature treatment. The contents of IAA and ZR in kernel were significantly reduced after being treated with high temperature. The content of GA3 was increased in inferior kernels, but not in superior kernels. It could be deduced that the much dry weight loss of inferior kernels should be ascribed to the increas-ing in GA3 content when kernels exposed to high temperature.