CAJ | 학술논문

从福建省邵武市的桃蚜上分离出1株病原真菌，通过形态学鉴定为西虫瘟霉 Zoophthora occidentalis。其初生分生孢子长椭圆形，有明显的孢领，大小为（23.4～31.2）μm ×（7.8～10.9）μm ， L/D ＝2.4～3.8。次生分生孢子与初生分生孢子同形，大小为（15.6～25.4）μm ×（7.8～10.4）μm ，L/D＝2.46～4.08。毛管孢子大小为（20.8～29.9）μm ×（6.0～10.4）μm ， L/D ＝2.4～3.6，柄长36.4～104.0μm。菌丝直径6.5～8.8μm。休眠孢子表面光滑，无色透明，直径23.9～29.9μm。用“孢子浴”方法将该菌对桃蚜接种，结果显示接种后第4 d为死亡高峰期，最终累计死亡率5.4％～95.7％，因剂量而异。经时间-剂量-死亡率模型分析，测得接种4 d后 L D50低于10个孢子· mm -2，表明该菌株对桃蚜具有较强的毒力。
종복건성소무시적도아상분리출1주병원진균，통과형태학감정위서충온매 Zoophthora occidentalis。기초생분생포자장타원형，유명현적포령，대소위（23.4～31.2）μm ×（7.8～10.9）μm ， L/D ＝2.4～3.8。차생분생포자여초생분생포자동형，대소위（15.6～25.4）μm ×（7.8～10.4）μm ，L/D＝2.46～4.08。모관포자대소위（20.8～29.9）μm ×（6.0～10.4）μm ， L/D ＝2.4～3.6，병장36.4～104.0μm。균사직경6.5～8.8μm。휴면포자표면광활，무색투명，직경23.9～29.9μm。용“포자욕”방법장해균대도아접충，결과현시접충후제4 d위사망고봉기，최종루계사망솔5.4％～95.7％，인제량이이。경시간-제량-사망솔모형분석，측득접충4 d후 L D50저우10개포자· mm -2，표명해균주대도아구유교강적독력。
A newly discovered fungal pathogen isolated from the green peach aphids ,Myzus persicae ,in Fujian was identified as Zoophthora occidentalis . Its elongate ovoid primary conidia sized (23.4 -31.2) μm × (7.8 -10.9)μm (L/D = 2.4-3.8) and had a clear spore collar .Secondary conidia liked primary (15.6-25.4) μm × (7.8-10.4) μm (L/D = 2.46 -4.08) was also oblong in shape (15.6 -25.4) μm × (7.8 -10.4) μm with an L/D ratio of 2.46-4.08 . The size of its capilliconidium was (20.8 -29.9) μm × (6.0 -10.4) μm (L/D = 2.4 -3.6) ,and the stipe was 36.4-104.0 μm long . The diameter of its mycelium was 6.5-8.8 μm . Its resting spores were smooth ,colorless and transparent with a diameter ranging from 23.9 μm to 29.9 μm . Most of the infected aphids were killed in the 4th day , and green peach aphids to induce a peaked death rate in 4 days and cumulative mortality ranging from 5.4% to 95.7% ,which increased with the spore concentration . The resulting data fitted well on a time-dose-mortality model . The LD50 after 4 days estimated by the model was less than 10 conidias · mm -2 ,indicating the entomophthoralean fungus to be a potent pathogen for M . persicae .