CAJ | 학술논문

采用正交试验对菌株T LB15的摇瓶发酵培养基进行了优化，结果表明：优化后的培养基为玉米粉3％、豆粉2％、蔗糖1％、硫酸铵0.1％、磷酸氢二钾0.3％、磷酸二氢钾0.2％、七水硫酸镁0.04％。采用10 L小型发酵罐发酵48 h后活菌数达4.52×109 cfu · mL -1，产芽孢率为90％。室内生物测定结果表明，菌株 TLB15发酵液在药后72 h对小菜蛾2龄幼虫和甜菜夜蛾2龄幼虫的校正死亡率为66.93％和69.07％。
채용정교시험대균주T LB15적요병발효배양기진행료우화，결과표명：우화후적배양기위옥미분3％、두분2％、자당1％、류산안0.1％、린산경이갑0.3％、린산이경갑0.2％、칠수류산미0.04％。채용10 L소형발효관발효48 h후활균수체4.52×109 cfu · mL -1，산아포솔위90％。실내생물측정결과표명，균주 TLB15발효액재약후72 h대소채아2령유충화첨채야아2령유충적교정사망솔위66.93％화69.07％。
The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37 ) . The results showed that the optimum fermentation culture medium contained 3.0% corm meal ,2.0% soybean powder , 1.0% sugar , 0.1% ammonium sulfate , 0.3% potassium hydrogen phosphate trihydrate ,0.2% potassium dihydrogen phosphate ,and 0.04% magnesium sulfate heptahydrate .The number of viable cells was 4.52 × 109 cfu · mL -1 and the rate of spore formation was 90% after 48hs′fermentation .The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua w ere 66.93% and 69.07% ,respectively .