国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2014年
10期
673-676
,共4页
乳腺肿瘤%瘦素%细胞凋亡%基因,Bcl-2
乳腺腫瘤%瘦素%細胞凋亡%基因,Bcl-2
유선종류%수소%세포조망%기인,Bcl-2
Breast neoplasms%Leptin%Apoptosis%Genes,Bcl-2
目的 探讨瘦素在体外对乳腺癌MCF-7细胞的凋亡抑制作用及其抑制MCF-7细胞凋亡的相关机制.方法 将MCF-7细胞接种于48孔板中.分组处理48 h后,ELISA测定不同浓度瘦素对不同浓度三苯氧胺引起的MCF-7细胞凋亡.实时荧光定量PCR方法检测各组细胞中Survivin、BCL-2、BAX的mRNA的相对表达水平改变.结果 103 ng/mL和104 ng/mL瘦素可以有效抑制TAM诱导的MCF-7细胞凋亡.104 ng/mL明显上调了乳腺癌MCF-7内Survivin mRNA的表达,而BCL-2、BAX mRNA改变无统计学意义.结论 瘦素可以有效抑制TAM诱导的MCF-7细胞凋亡,其机制可能与瘦素上调Survivin mRNA的表达有关.
目的 探討瘦素在體外對乳腺癌MCF-7細胞的凋亡抑製作用及其抑製MCF-7細胞凋亡的相關機製.方法 將MCF-7細胞接種于48孔闆中.分組處理48 h後,ELISA測定不同濃度瘦素對不同濃度三苯氧胺引起的MCF-7細胞凋亡.實時熒光定量PCR方法檢測各組細胞中Survivin、BCL-2、BAX的mRNA的相對錶達水平改變.結果 103 ng/mL和104 ng/mL瘦素可以有效抑製TAM誘導的MCF-7細胞凋亡.104 ng/mL明顯上調瞭乳腺癌MCF-7內Survivin mRNA的錶達,而BCL-2、BAX mRNA改變無統計學意義.結論 瘦素可以有效抑製TAM誘導的MCF-7細胞凋亡,其機製可能與瘦素上調Survivin mRNA的錶達有關.
목적 탐토수소재체외대유선암MCF-7세포적조망억제작용급기억제MCF-7세포조망적상관궤제.방법 장MCF-7세포접충우48공판중.분조처리48 h후,ELISA측정불동농도수소대불동농도삼분양알인기적MCF-7세포조망.실시형광정량PCR방법검측각조세포중Survivin、BCL-2、BAX적mRNA적상대표체수평개변.결과 103 ng/mL화104 ng/mL수소가이유효억제TAM유도적MCF-7세포조망.104 ng/mL명현상조료유선암MCF-7내Survivin mRNA적표체,이BCL-2、BAX mRNA개변무통계학의의.결론 수소가이유효억제TAM유도적MCF-7세포조망,기궤제가능여수소상조Survivin mRNA적표체유관.
Objective To investigate inhibition effect of leptin on apoptosis of MCF-7 cells and its potential mechanism.Methods MCF-7 cells were cultured and devided into 5 groups:control group,tamoxifen (10-5 mol/L) group,tamoxifen(10-5 mol/L)-leptin(102 ng/mL)group,tamoxifen (10-5 mol/L)-leptin(103 ng/mL) group,tamoxifen(10-5 mol/L)-leptin(104 ng/mL)group.Fourty-eight hours after being treated with corresponding concentration of leptin and tamoxifen respectively,all groups of cells were quantatively tested for apoptosis by ELISA and were semi-quantatively detected for survivin,Bcl-2,Bax mRNA change by real-time PCR.Results Tamoxifen induced MCF-7 apoptosis were inhibited by leptin at a serial concentration of 103 ng/mL,104 ng/mL.Meanwhile,leptin largely enhanced survivin mRNA expression in MCF-7 cells,compared with control group.However,no significant increase of BCL-2,BAX mRNA in MCF-7 cells was observed after leptin treatment in either group.Conclusions Leptin could effectively inhibit tamoxifen induced MCF-7 apoptosis in a dose dependent manner.The potential mechanisrn of leptin' apoptosis inbition in MCF-7 cells may involve upregulation of survivin mRNA.
