CAJ | 학술논문

以杜仲叶渣为载体，巨大芽孢杆菌为发酵菌种，以其活菌数为指标，通过正交试验考察了杜仲叶渣固态发酵制备解磷生物肥料的最佳工艺条件。结果表明，杜仲叶渣制备解磷生物肥料的最佳工艺条件为：料液比（质量和体积比）1∶2．5，料层厚度6 cm，接种量（每毫升细菌数量为8．56×109个）1．5 mL，发酵温度30℃，杜仲叶渣与倍花废渣的质量比为1∶0．4。此培养条件下巨大芽孢杆菌的最大活菌数可达1．64×109 cfu/g。此外，由最佳工艺条件制备的解磷生物肥料，其相关指标均在国标限量内。因此，杜仲叶渣可以作为一种良好的载体来制备解磷生物肥料。
이두중협사위재체，거대아포간균위발효균충，이기활균수위지표，통과정교시험고찰료두중협사고태발효제비해린생물비료적최가공예조건。결과표명，두중협사제비해린생물비료적최가공예조건위：료액비（질량화체적비）1∶2．5，료층후도6 cm，접충량（매호승세균수량위8．56×109개）1．5 mL，발효온도30℃，두중협사여배화폐사적질량비위1∶0．4。차배양조건하거대아포간균적최대활균수가체1．64×109 cfu/g。차외，유최가공예조건제비적해린생물비료，기상관지표균재국표한량내。인차，두중협사가이작위일충량호적재체래제비해린생물비료。
In order to research optimal technological conditions of solid-state fermentation to prepare phosphate-solubile biological fertilizer, the leaves residual of Eucommia ulmoides Olive as carrier, Bacillus megaterium as fermented culture and viable count as index were employed by using the orthogonal test. The results showed that optimal technological con-ditions of phosphate-solubile biological fertilizer prepared by leaves residual of E. unmolds Olive was as follows:ratio of liquor to material was 1∶2.5, substrate thickness was 6 cm, the amount of inoculum was 1.5 mL, temperature was 30℃, and the mass ratio of leaves residual of E. ulmoides Olive and waste slag of floraphis was 1∶0.4. The maximum viable count of B. megaterium could reach 1.64×109 cfu/g at aforesaid experimental condition. In addition, contents of related indices of the phosphate-solubilizing biological fertilizer prepared by optimal technological conditions were all within the range of na-tional standard. As a result, leaves residual of E. ulmoides Olive could be used as carrier to prepare biological fertilizer.