CAJ | 학술논문

翻译起始因子eIF1A基因是蛋白合成的重要调控因子之一，在一些植物中可能参与抗逆调控过程。为了研究刚毛柽柳TheIF1A基因是否参与抗逆过程，将TheIF1A基因插入酵母表达载体pYES2中构建成重组载体，转入酿酒酵母（ Saccharomyces cerevisiae）中获得重组型酵母，分别比较转TheIF1A基因酵母和转空载体对照酵母在山梨醇、H2 O2、CdCl2、CuSO4、ZnCl2、KCl、Na2 CO3、MgCl2、-20℃和53℃胁迫处理之后的存活能力。结果显示， TheIF1A基因能有效提高转基因酵母的抗干旱、盐碱、氧化、重金属及极端温度的能力，表明TheIF1A可能参与了柽柳多种抗逆调控过程。
번역기시인자eIF1A기인시단백합성적중요조공인자지일，재일사식물중가능삼여항역조공과정。위료연구강모정류TheIF1A기인시부삼여항역과정，장TheIF1A기인삽입효모표체재체pYES2중구건성중조재체，전입양주효모（ Saccharomyces cerevisiae）중획득중조형효모，분별비교전TheIF1A기인효모화전공재체대조효모재산리순、H2 O2、CdCl2、CuSO4、ZnCl2、KCl、Na2 CO3、MgCl2、-20℃화53℃협박처리지후적존활능력。결과현시， TheIF1A기인능유효제고전기인효모적항간한、염감、양화、중금속급겁단온도적능력，표명TheIF1A가능삼여료정류다충항역조공과정。
Translation initiation factor 1A is an important regulation factor for protein synthesis, which may involve in plant abiotic stress regulation. In order to study the stress tolerance of a translation initiation factor 1A ( TheIF1A) from Tamarix hispida, the recombinant plasmid, named as pYES2-TheIF1A, was constructed by inserting the TheIF1A gene into the yeast expression vector pYES2. The plasmid pYES2-TheIF1A was transformed into the yeast Saccharomyces cere-visiae, and the yeast transformed with empty pYES2 was employed as a control. The pYES2-TheIF1A and pYES2 recom-binant yeast were respectively treated with sorbitol, H2 O2 , CdCl2 , CuSO4 , ZnCl2 , KCl, Na2 CO3 , MgCl2 , low tempera-ture -20℃ and high temperature 53℃, respectively. Results showed that the yeast expressing TheIF1A had greater tol-erant to salt, drought, heavy metals, and both low and high temperatures than the control, suggesting that TheIF1A gene might an important stress-regulating gene in T.hispida.