CAJ | 학술논문

质粒DNA含有独立复制的遗传结构，是基因工程的常用工具，广泛应用于分子生物学基础研究、农业转基因检测、医疗诊断与基因治疗等领域。质粒DNA的构型一般分为超螺旋、开口环状及线性3种。初步纯化的质粒DNA溶液中常混有3种构型，并且掺杂一定量的蛋白质、RNA、内毒素以及宿主基因组DNA，这些杂质会影响后续的应用，因此质粒DNA需要进一步精细纯化。该文对质粒DNA的新应用领域、基于色谱的精细纯化技术及产物质量分析体系进行了综述，并展望了高纯度质粒DNA精细纯化及产物分析的发展方向。
질립DNA함유독립복제적유전결구，시기인공정적상용공구，엄범응용우분자생물학기출연구、농업전기인검측、의료진단여기인치료등영역。질립DNA적구형일반분위초라선、개구배상급선성3충。초보순화적질립DNA용액중상혼유3충구형，병차참잡일정량적단백질、RNA、내독소이급숙주기인조DNA，저사잡질회영향후속적응용，인차질립DNA수요진일보정세순화。해문대질립DNA적신응용영역、기우색보적정세순화기술급산물질량분석체계진행료종술，병전망료고순도질립DNA정세순화급산물분석적발전방향。
Plasmid DNA,a common tool of genetic engineering containing the independent genetic structure of the replication,is widely used in basic research of molecular biology,genetically modi-fied detection,medical diagnosis and gene therapy. Plasmid DNA conformations are generally divided into three types:supercoiled,open circle and linear. Crude plasmid DNA often mixes with three configurations,and the amount of protein,RNA,endotoxin with the host genome DNA. These im-purities need to be further refined as they affect subsequent applications. The new applications of plasmid DNA,refined technologies and products based on chromatography system,and quality anal-ysis of purified plasmid DNA is reviewed in this paper. Finally,the trends of technology of high pu-rity plasmid DNA and analysis of refined product are presented.