分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2014年
9期
1083-1088
,共6页
禁用着色剂%化妆品%高效液相色谱
禁用著色劑%化妝品%高效液相色譜
금용착색제%화장품%고효액상색보
forbidden colorants%cosmetics%high performance liquid chromatography(HPLC)
采用高效液相色谱建立了同时测定化妆品中14种禁用着色剂（吖啶黄、溶剂蓝35、溶剂红49、酸性紫49、氯化四甲基副玫瑰苯胺、颜料橙5、颜料红53、氯化五甲基副玫瑰苯胺、苏丹红Ⅱ、氯化六甲基副玫瑰苯胺、苏丹红Ⅳ、罗丹明B、分散黄3、苏丹红Ⅰ）的分析方法。待测化妆品样品经四氢呋喃分散，并对目标物进行提取后，用含有醋酸铵的甲醇-水混合溶液将基质析出，提取液经离心过滤后，以10 mmol/L的醋酸铵溶液-乙腈作为流动相在Agilent poroshell 120 EC-C18（2.7μm，3.0 mm×100 mm）色谱柱上梯度洗脱进行分离，检测波长为416，514，590 nm。以目标物的色谱保留时间和紫外光谱图进行定性，以色谱峰的峰面积用标准曲线外标法进行定量。在优化条件下，各目标物的线性范围为0.2~20μg/mL，相关系数均大于0.999。14种禁用着色剂的定量下限为3~10μg/g。在低、中、高3个加标水平下，各目标物的回收率为91.3％~110.9％，相对标准偏差（ RSD）均低于10％。方法准确、简便、灵敏、可靠，可用于化妆品中此14种禁用着色剂的定量测定。
採用高效液相色譜建立瞭同時測定化妝品中14種禁用著色劑（吖啶黃、溶劑藍35、溶劑紅49、痠性紫49、氯化四甲基副玫瑰苯胺、顏料橙5、顏料紅53、氯化五甲基副玫瑰苯胺、囌丹紅Ⅱ、氯化六甲基副玫瑰苯胺、囌丹紅Ⅳ、囉丹明B、分散黃3、囌丹紅Ⅰ）的分析方法。待測化妝品樣品經四氫呋喃分散，併對目標物進行提取後，用含有醋痠銨的甲醇-水混閤溶液將基質析齣，提取液經離心過濾後，以10 mmol/L的醋痠銨溶液-乙腈作為流動相在Agilent poroshell 120 EC-C18（2.7μm，3.0 mm×100 mm）色譜柱上梯度洗脫進行分離，檢測波長為416，514，590 nm。以目標物的色譜保留時間和紫外光譜圖進行定性，以色譜峰的峰麵積用標準麯線外標法進行定量。在優化條件下，各目標物的線性範圍為0.2~20μg/mL，相關繫數均大于0.999。14種禁用著色劑的定量下限為3~10μg/g。在低、中、高3箇加標水平下，各目標物的迴收率為91.3％~110.9％，相對標準偏差（ RSD）均低于10％。方法準確、簡便、靈敏、可靠，可用于化妝品中此14種禁用著色劑的定量測定。
채용고효액상색보건립료동시측정화장품중14충금용착색제（아정황、용제람35、용제홍49、산성자49、록화사갑기부매괴분알、안료등5、안료홍53、록화오갑기부매괴분알、소단홍Ⅱ、록화륙갑기부매괴분알、소단홍Ⅳ、라단명B、분산황3、소단홍Ⅰ）적분석방법。대측화장품양품경사경부남분산，병대목표물진행제취후，용함유작산안적갑순-수혼합용액장기질석출，제취액경리심과려후，이10 mmol/L적작산안용액-을정작위류동상재Agilent poroshell 120 EC-C18（2.7μm，3.0 mm×100 mm）색보주상제도세탈진행분리，검측파장위416，514，590 nm。이목표물적색보보류시간화자외광보도진행정성，이색보봉적봉면적용표준곡선외표법진행정량。재우화조건하，각목표물적선성범위위0.2~20μg/mL，상관계수균대우0.999。14충금용착색제적정량하한위3~10μg/g。재저、중、고3개가표수평하，각목표물적회수솔위91.3％~110.9％，상대표준편차（ RSD）균저우10％。방법준학、간편、령민、가고，가용우화장품중차14충금용착색제적정량측정。
A high performance liquid chromatographic( HPLC)methord was developed for the simul-taneous determination of 14 forbidden colorants( Metanil Yellow,Solvent Blue 35,Solvent Red 49, Acid Violet 49,Tetramethylpararosaniline Chloride( TMPC),Pigment Orange 5,Pigment Red 53, Pentamethyltriphenylmethane( PMTPE ), Sudan Red Ⅱ, Hexamethylenepararosaniline Chloride (HMPC),Solvent Red 24,Rhodamine B,Disperse Yellow 3,Sudan Red Ⅰ)in cosmetics. Cos-metic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after additon of ammonium acetate solution and methanol. After centrifuged and filtered,the ex-tract was separated on an Agilent poroshell 120 EC-C18 column(2. 7 μm,3. 0 mmí100 mm)by a gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase. Detection wave-lengths were set at 416,514,590 nm,respectively. The qualitative analysis was based on chroma-tographic retention time and UV spectrum,and the quantitative analysis was performed by the stand-ard curve method of peak area. The linear ranges of 14 analytes were in the range of 0. 2-20 μg/mL,with correlation coefficients more than 0. 999. The limits of quantitation( LOQs)were in the range of 3-10 μg/g. The average recoveries at three spiked levels ranged from 91. 3% to 110. 9%with RSDs less than 10% . This method was accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.
