CAJ | 학술논문

采用正交实验方法对聚焦微波辅助提取（ FMAE）的实验条件进行优化，以天芥菜碱为提取目标物， HPLC法测定其含量。通过优化，得到最佳实验条件：药材粒度60目，液料比1：35，微波功率420 W，微波时间50 min。HPLC分析条件：以甲醇-0.05％三乙胺（ pH 7.5）（30：70）为流动相，在220 nm处检测，天芥菜碱的线性范围为52~1040 mg/L，相关系数（ r）为0.9999，检出限为0.0402 mg/L，其加标回收率为97.5％~103％，相对标准偏差为2.4％，方法简单、灵敏度高。
채용정교실험방법대취초미파보조제취（ FMAE）적실험조건진행우화，이천개채감위제취목표물， HPLC법측정기함량。통과우화，득도최가실험조건：약재립도60목，액료비1：35，미파공솔420 W，미파시간50 min。HPLC분석조건：이갑순-0.05％삼을알（ pH 7.5）（30：70）위류동상，재220 nm처검측，천개채감적선성범위위52~1040 mg/L，상관계수（ r）위0.9999，검출한위0.0402 mg/L，기가표회수솔위97.5％~103％，상대표준편차위2.4％，방법간단、령민도고。
An extraction method of heliotrine was developed by focused microwave-assisted extraction ( FMAE). Orthogonal design was used to optimize the experimental conditions. The optimum extrac-tion conditions were as follows:60 mesh size of material,solvent-materail ratio:1 : 35,micro-wave power:420 W and extraction time:50 min. The analyte was determined by HPLC using meth-anol -0. 05% triethylamine(pH 7. 5 )(30 : 70)as mobile phase with a detection wavelength of 220 nm. The linear range of heliotrine was in the range of 52-1 040 mg/L(r=0. 999 9),with a detec-tion limit of 0. 040 2 mg/L. The recoveries for heliotrine were in the range of 97. 5% -103% with RSDs of 2. 4% . The method was simple and highly sensitive.