CAJ | 학술논문

首次报道了博来霉素（BLM）与亚铁离子相互结合，形成的BLM·Fe（Ⅱ）复合物具有内在的过氧化物模拟酶催化活性，能够催化过氧化氢氧化2，2’-联氮基-双（3-乙基苯并噻唑-6-磺酸）（ABTS）的显色反应，产生深绿色的产物。与天然酶辣根过氧化物酶类似，BLM·Fe（Ⅱ）复合物的催化活性强烈依赖于pH值和温度，相应的最优化条件分别为pH 6.0和30℃。利用BLM·Fe（Ⅱ）复合物催化ABTS的显色反应，建立了一种简便快捷的可视化检测博来霉素的新方法，检出限可达14.1 nmol/L。该检测方法还显示了良好的可重复性和选择性，在临床分析中具有潜在的应用价值。
수차보도료박래매소（BLM）여아철리자상호결합，형성적BLM·Fe（Ⅱ）복합물구유내재적과양화물모의매최화활성，능구최화과양화경양화2，2’-련담기-쌍（3-을기분병새서-6-광산）（ABTS）적현색반응，산생심록색적산물。여천연매랄근과양화물매유사，BLM·Fe（Ⅱ）복합물적최화활성강렬의뢰우pH치화온도，상응적최우화조건분별위pH 6.0화30℃。이용BLM·Fe（Ⅱ）복합물최화ABTS적현색반응，건립료일충간편쾌첩적가시화검측박래매소적신방법，검출한가체14.1 nmol/L。해검측방법환현시료량호적가중복성화선택성，재림상분석중구유잠재적응용개치。
Bleomycins( BLMs),a family of glycopeptide-derived antibiotics,are widely used as a-vailable chemotherapeutic agents against a variety of cancers. In the presence of Fe(Ⅱ)ions,BLM form a binary BLM·Fe(Ⅱ)complex that can activate molecular oxygen. Herein,it is demonstrate for the first time that BLM · Fe(Ⅱ)complex may possess an intrinsic peroxidase-like activity and could catalyze 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)into colored product in the presence of hydrogen peroxide. Experimental results revealed that the catalytic activity of BLM· Fe(Ⅱ)complex is strongly dependent on pH value and temperature,which is similar to natural en-zymes such as horseradish peroxidase. The conditions for maximum catalytic efficiency were deter-mined to be pH 6. 0 and 30 ℃. Taking advantage of the color reaction of ABTS catalyzed by BLM· Fe(Ⅱ)complex,a simple and rapid colorimetric method for the detection of BLM was thus estab-lished. Under the optimal conditions,a good linear relationship was obtained over BLM concentra-tion in the range of 0. 1-2. 5 μmol/L with a detection limit of 14. 1 nmol/L. With the advantages of good reproducibility and high selectivity,the method is anticipated to have a practical potential for BLM analysis in clinical applications.