分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2014年
9期
1038-1043,1049
,共7页
二阶校正法%三维荧光%内滤光效应%吸光度校正%槲皮素
二階校正法%三維熒光%內濾光效應%吸光度校正%槲皮素
이계교정법%삼유형광%내려광효응%흡광도교정%곡피소
second-order calibration%3 D fluorescence%inner filter effects%absorbance correction%quercetin
采用三维荧光并结合二阶校正的平行因子分析（ PARAFAC）算法、交替三线性（ ATLD）算法和自加权交替三线性（ SWATLD）算法对中药绞股蓝中的槲皮素含量和回收率进行测定，Al（Ⅲ）作敏化剂增加槲皮素的荧光强度。核一致检验法说明体系中荧光组分数为2，其中一个组分对应于槲皮素，另一个对应于中药中的一个共存组分。3种方法测定中药绞股蓝中槲皮素的含量分别为0.2749％，0.2729％和0.2727％，回收率分别为96.3％，103.1％和96.8％，得到的槲皮素解析光谱和真实光谱几乎完全重合。实验结果表明，化学计量学“二阶校正”法具有简单、快速、高效及操作费用低等特点，可用于复杂成分的定性和定量分析。同时，还讨论了中药溶液中内滤光效应对荧光强度及定量分析的影响，并采用数学校正法对中药溶液的内滤光效应进行校正，实验发现测定的荧光强度用相应的溶液吸光度校正后，定量分析结果的准确度大大改善。
採用三維熒光併結閤二階校正的平行因子分析（ PARAFAC）算法、交替三線性（ ATLD）算法和自加權交替三線性（ SWATLD）算法對中藥絞股藍中的槲皮素含量和迴收率進行測定，Al（Ⅲ）作敏化劑增加槲皮素的熒光彊度。覈一緻檢驗法說明體繫中熒光組分數為2，其中一箇組分對應于槲皮素，另一箇對應于中藥中的一箇共存組分。3種方法測定中藥絞股藍中槲皮素的含量分彆為0.2749％，0.2729％和0.2727％，迴收率分彆為96.3％，103.1％和96.8％，得到的槲皮素解析光譜和真實光譜幾乎完全重閤。實驗結果錶明，化學計量學“二階校正”法具有簡單、快速、高效及操作費用低等特點，可用于複雜成分的定性和定量分析。同時，還討論瞭中藥溶液中內濾光效應對熒光彊度及定量分析的影響，併採用數學校正法對中藥溶液的內濾光效應進行校正，實驗髮現測定的熒光彊度用相應的溶液吸光度校正後，定量分析結果的準確度大大改善。
채용삼유형광병결합이계교정적평행인자분석（ PARAFAC）산법、교체삼선성（ ATLD）산법화자가권교체삼선성（ SWATLD）산법대중약교고람중적곡피소함량화회수솔진행측정，Al（Ⅲ）작민화제증가곡피소적형광강도。핵일치검험법설명체계중형광조분수위2，기중일개조분대응우곡피소，령일개대응우중약중적일개공존조분。3충방법측정중약교고람중곡피소적함량분별위0.2749％，0.2729％화0.2727％，회수솔분별위96.3％，103.1％화96.8％，득도적곡피소해석광보화진실광보궤호완전중합。실험결과표명，화학계량학“이계교정”법구유간단、쾌속、고효급조작비용저등특점，가용우복잡성분적정성화정량분석。동시，환토론료중약용액중내려광효응대형광강도급정량분석적영향，병채용수학교정법대중약용액적내려광효응진행교정，실험발현측정적형광강도용상응적용액흡광도교정후，정량분석결과적준학도대대개선。
A simple,rapid and effective method for the quantitative analysis of quercetin in Gynos-temma pentaphyllum was established by using excitation-emission matrix( EEM)fluorescence cou-pled with second-order calibration based on Parallel factor analysis( PARAFAC)algorithm,alterna-ting trilinear decomposition( ATLD)algorithm and self-weighted alternating trilinear decomposition ( SWATLD)algorithm. Al(Ⅲ)was used as fluorescence sensitizer to increase the quercetin fluores-cence intensity. The core-consistency diagnostic( CORCONDIA)test indicated the number of compo-nents should be two,one of the components corresponds to the quercetin,and the other is related to a coexistence component of traditional Chinese medicine. The content of quercetin by PARAFAC, ATLD,SWATLD with component number N =2 are 0. 274 9% ,0. 272 9% and 0. 272 7% ,and the average recoveries were 96. 3% ,103. 1% and 96. 8% , respectively. The resolved quercetin spectra overlap almost completely with real spectra. The experimental results showed that the chemo-metrics“second order correction”method was simple,rapid,high efficient and low cost,and could be used for complex component qualitative and quantitative analysis. In addition,the influence of the inner filter effect on fluorescence intensity was discussed,and the results revealed that ultraviolet cor-rection could effectively improve the accuracy of quantitative analysis.
