分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2014年
9期
1019-1025
,共7页
冯利彬%齐崴%苏荣欣%何志敏
馮利彬%齊崴%囌榮訢%何誌敏
풍리빈%제외%소영흔%하지민
聚(三聚氰胺)%金纳米粒子%芦丁%示差脉冲伏安法
聚(三聚氰胺)%金納米粒子%蘆丁%示差脈遲伏安法
취(삼취청알)%금납미입자%호정%시차맥충복안법
poly-( melamine)%gold nonoparticles%rutin%differential pulse voltammetry
通过电聚合和电沉积方法首次制得聚（三聚氰胺）和金纳米粒共修饰的电极（ PMel/Au/GCE），并对修饰电极进行交流阻抗电化学分析。采用循环伏安法研究了芦丁在修饰电极上的电化学行为，发现其氧化峰电流和还原峰电流较裸玻碳电极（ GCE）以及聚（三聚氰胺）修饰的电极（ PMel/GCE）明显增强，提高了检测的灵敏度。对溶液的pH值、金纳米粒子电沉积时间、三聚氰胺电聚合时间和扫描速率等实验条件进行了优化。采用示差脉冲伏安法对芦丁进行定量分析，芦丁浓度分别在7.8×10-9~1.2×10-6 mol/L和1.2×10-6~1.5×10-5 mol/L范围内与峰电流呈线性，其相关系数（ r2）分别为0.997和0.993，检出限（ S/N=3）为5.5×10-9 mol/L。将该电极用于市售芦丁片检测，回收率为96.4％~101.8％。
通過電聚閤和電沉積方法首次製得聚（三聚氰胺）和金納米粒共脩飾的電極（ PMel/Au/GCE），併對脩飾電極進行交流阻抗電化學分析。採用循環伏安法研究瞭蘆丁在脩飾電極上的電化學行為，髮現其氧化峰電流和還原峰電流較裸玻碳電極（ GCE）以及聚（三聚氰胺）脩飾的電極（ PMel/GCE）明顯增彊，提高瞭檢測的靈敏度。對溶液的pH值、金納米粒子電沉積時間、三聚氰胺電聚閤時間和掃描速率等實驗條件進行瞭優化。採用示差脈遲伏安法對蘆丁進行定量分析，蘆丁濃度分彆在7.8×10-9~1.2×10-6 mol/L和1.2×10-6~1.5×10-5 mol/L範圍內與峰電流呈線性，其相關繫數（ r2）分彆為0.997和0.993，檢齣限（ S/N=3）為5.5×10-9 mol/L。將該電極用于市售蘆丁片檢測，迴收率為96.4％~101.8％。
통과전취합화전침적방법수차제득취（삼취청알）화금납미립공수식적전겁（ PMel/Au/GCE），병대수식전겁진행교류조항전화학분석。채용순배복안법연구료호정재수식전겁상적전화학행위，발현기양화봉전류화환원봉전류교라파탄전겁（ GCE）이급취（삼취청알）수식적전겁（ PMel/GCE）명현증강，제고료검측적령민도。대용액적pH치、금납미입자전침적시간、삼취청알전취합시간화소묘속솔등실험조건진행료우화。채용시차맥충복안법대호정진행정량분석，호정농도분별재7.8×10-9~1.2×10-6 mol/L화1.2×10-6~1.5×10-5 mol/L범위내여봉전류정선성，기상관계수（ r2）분별위0.997화0.993，검출한（ S/N=3）위5.5×10-9 mol/L。장해전겁용우시수호정편검측，회수솔위96.4％~101.8％。
An electrode modified by gold nanoparticles( AuNPs)and polymerized film of melamine (PMel)(PMel/Au/GCE)was fabricated. Electrochemical impedance spectroscopy(EIS)was used for analyzing the modified electrode. The electrochemical behavior of rutin was investigated by cyclic voltammetry( CV),and a couple of sensitive and reversible redox peaks were obtained with an oxida-tion peak at 0. 422 V and a reduction peak at 0. 374 V. It was found that the oxidation peak current ( Ipa )and the reductive peak( Ipc )current at PMel/Au/GCE were obviously larger than those at bare glassy carbon electrode( GCE )and the electrode modified by polymerized film of melamine( PMel/GCE),which indicated that the sensitivity for detection of rutin was improved. Experimental param-eters,including pH value of buffer solution,electro-deposited time of AuNPs,electro-polymerized time of PMel and scan rate were optimized. The differential pulse voltammetry( DPV)was used for the quantitative analysis on rutin. Good linear relationships between current intensities and rutin con-centrations were obtained in the ranges of 7. 8 í10 -9 -1. 2 í10 -6 mol/L and 1. 2 í10 -6 -1. 5 í10 -5mol/L,with correlation coefficients(r2)of 0. 997 and 0. 993,respectively. The detection limit (S/N=3)was 5. 5í10 -9 mol/L. The modified electrode was applied in the determination of rutin in rutin tablets with recoveries of 96. 4% -101. 8% .
