四川医学
四川醫學
사천의학
SICHUAN MEDICAL JOURNAL
2014年
8期
955-956,957
,共3页
TGFβ2%基因单核苷酸多态性%持续性心房颤动
TGFβ2%基因單覈苷痠多態性%持續性心房顫動
TGFβ2%기인단핵감산다태성%지속성심방전동
TGFβ2%single nucleotide polymorphism%Persistent atrial fibrillation
目的：通过研究转移生长因子β2(TGFβ2)基因单核苷酸多态性与持续性心房颤动(AF)的相关性,探讨AF的免疫遗传学发病机制。方法从外周血中提取DNA,采用聚合酶链扩增反应-限制性片段长度多态性技术检测90例西南地区人群AF患者及90例健康对照者TGFβ2基因一个单核苷酸标签位点(rs6658835)多态性。用检验比较病例组与对照组之间基因型频率和等位基因频率的统计学差异。结果 AF组与对照组基因型分布均符合Hardy-Weinberg平衡。与正常对照组相比,AF患者组TGFβ2基因标签位点(rs6658835)GG+AG基因型和G等位基因频率明显增加,差异有统计学意义(分别为83．4% vs.65．6%和60．6% vs.41．7%,P均<0．05)。结论本研究发现TGFβ2基因rs6658835位点单核苷酸多态性与西南地区人群AF相关。 TGFβ2基因多态性可能在AF遗传易感性方面具有重要作用。
目的：通過研究轉移生長因子β2(TGFβ2)基因單覈苷痠多態性與持續性心房顫動(AF)的相關性,探討AF的免疫遺傳學髮病機製。方法從外週血中提取DNA,採用聚閤酶鏈擴增反應-限製性片段長度多態性技術檢測90例西南地區人群AF患者及90例健康對照者TGFβ2基因一箇單覈苷痠標籤位點(rs6658835)多態性。用檢驗比較病例組與對照組之間基因型頻率和等位基因頻率的統計學差異。結果 AF組與對照組基因型分佈均符閤Hardy-Weinberg平衡。與正常對照組相比,AF患者組TGFβ2基因標籤位點(rs6658835)GG+AG基因型和G等位基因頻率明顯增加,差異有統計學意義(分彆為83．4% vs.65．6%和60．6% vs.41．7%,P均<0．05)。結論本研究髮現TGFβ2基因rs6658835位點單覈苷痠多態性與西南地區人群AF相關。 TGFβ2基因多態性可能在AF遺傳易感性方麵具有重要作用。
목적：통과연구전이생장인자β2(TGFβ2)기인단핵감산다태성여지속성심방전동(AF)적상관성,탐토AF적면역유전학발병궤제。방법종외주혈중제취DNA,채용취합매련확증반응-한제성편단장도다태성기술검측90례서남지구인군AF환자급90례건강대조자TGFβ2기인일개단핵감산표첨위점(rs6658835)다태성。용검험비교병례조여대조조지간기인형빈솔화등위기인빈솔적통계학차이。결과 AF조여대조조기인형분포균부합Hardy-Weinberg평형。여정상대조조상비,AF환자조TGFβ2기인표첨위점(rs6658835)GG+AG기인형화G등위기인빈솔명현증가,차이유통계학의의(분별위83．4% vs.65．6%화60．6% vs.41．7%,P균<0．05)。결론본연구발현TGFβ2기인rs6658835위점단핵감산다태성여서남지구인군AF상관。 TGFβ2기인다태성가능재AF유전역감성방면구유중요작용。
Objective To investigate the association of the single nucleotide polymorphism( SNP) of transforming growth fac-tor β2(TGFβ2)gene and Persistent atrial fibrillation(AF)and to explore genetic mechanism of AF caused possibly by TGFβ2 gene polymorphism. Methods The sample DNA was extracted from peripheral blood of AF patients in the population of Southwestern Chi-na(n=90)and normal control group(n=90). The SNP of TGFβ2 gene at one tag position(rs6658835) was determined by using the analysis techniques of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism ( PCR-RFLP). The genotype frequen-cies and the allele frequencies of SNP were compared with test in two groups. Results Genotype distributions had no deviation from Hardy-Weinberg equilibrium both in AF patients and control subjects. Compared with controls, the frequency of GG and AG genotypes(83. 4% vs. 65. 6%,P<0. 05)and the frequency of G allele(60. 6% vs. 41. 7%,P<0. 05)of the SNP at the tag position (rs6658835)are apparently increased in AF patients. There was significant difference in the frequency distribution of genotype and allele in rs6658835 between two groups. Conclusion Our study find SNP rs6658835 in TGFβ2 gene has association with AF in the population of Southwestern China. TGFβ2 gene polymorphisms may play an important role in the susceptibility of AF.
