CAJ | 학술논문

目的：检测淋球菌多重耐药株的MtrR基因及FarAB基因的点突变位点,比较二者耐药基因及耐药底物的差异。方法收集淋病患者分泌物标本分离出各型耐药淋球菌株,PCR扩增各菌株中MtrR基因、FarAB基因并测序。结果各型淋球菌耐药株中检测出MtrR,FarAB基因不同突变方式。结论淋球菌多重耐药菌株有Mtr基因、FarAB基因突变造成了淋球菌的不同程度的耐药性能,增加了淋球菌对Has的耐受性,对改变临床抗生素用药具有重要指导意义。
목적：검측림구균다중내약주적MtrR기인급FarAB기인적점돌변위점,비교이자내약기인급내약저물적차이。방법수집임병환자분비물표본분리출각형내약림구균주,PCR확증각균주중MtrR기인、FarAB기인병측서。결과각형림구균내약주중검측출MtrR,FarAB기인불동돌변방식。결론림구균다중내약균주유Mtr기인、FarAB기인돌변조성료림구균적불동정도적내약성능,증가료림구균대Has적내수성,대개변림상항생소용약구유중요지도의의。
Objective To explore and find out the Mtr and FarAB gene mutation locus in multiple resistances system of Nesseria gonorrhoeae, and compare the differences of the two gene mutation and resistant substrate. Methods The Gonorrhea pa-tients’ secretion was collected and isolated out kinds of resistant strains by drug sensitive test. Mtr and FarAB gene of in each strain were amplificated by PCR and analyzed to find out mutation locus. Results These findings showed that every resistant strain has an unique mutation manner in Mtr and FarAB gene. Conclusions The mutations detected in Mtr and FarAB gene of neis-seria gonorrhoeae were responsible for the multiple drug-resistant capacities and were important to guidance of clinical antibiotic treat-ment.