CAJ | 학술논문

对蔬菜水果样品中的赤霉素残留量采用高效液相色谱方法进行测定。结果表明：样品中赤霉素经80%甲醇溶液提取,用石油醚脱色,经过乙酸乙酯液液萃取和磷酸缓冲液反萃取,再用乙酸乙酯萃取净化,然后采用C18色谱柱分离,以甲醇和0.1%的冰乙酸水溶液为流动相洗脱,用高效液相色谱紫外检测器206 nm 波长检测,以保留时间定性,外标法定量。在此优化实验条件下,赤霉素标准溶液在0.25～50 mg/L 呈良好线性, r＝0.9999,检出限为0.067 mg/kg。6种蔬菜水果的添加回收率为71.6%～105.2%, RSD 为2.1%～9.8%。
대소채수과양품중적적매소잔류량채용고효액상색보방법진행측정。결과표명：양품중적매소경80%갑순용액제취,용석유미탈색,경과을산을지액액췌취화린산완충액반췌취,재용을산을지췌취정화,연후채용C18색보주분리,이갑순화0.1%적빙을산수용액위류동상세탈,용고효액상색보자외검측기206 nm 파장검측,이보류시간정성,외표법정량。재차우화실험조건하,적매소표준용액재0.25～50 mg/L 정량호선성, r＝0.9999,검출한위0.067 mg/kg。6충소채수과적첨가회수솔위71.6%～105.2%, RSD 위2.1%～9.8%。
Researched the analytical method for the determination of gibberellin acid residue in vegetables and fruit by high-performance liquid chromatography. The GA3 , after being extrated by using 80% methoanol water solution, decolored by petroleum ether, extracted by ethyl acetace, and then, purged by reversed extraction with Phosphate Buffer (PB) and extrated with ethyl acetate again, was separated on C18 column using methonl and 0.1% acetic acid solution as mobile phases and 206 nm as HPLC detected wavelength. The samples were qualitatived by retention time and quantified with the external calibration method. The results showed the method performed well within the linear range between 0.25 mg/L and 50 mg/L (r=0.999 9), and the limit of detection was 0.067 mg/kg. The method was used to detect the contents of GA3 in 6 kinds of vegetables and fruit, the recoveries for the spiked samples were ranged from 71.6% to 105.2%, and the variation coefficients were from 2.1% to 9.8%.