CAJ | 학술논문

为获得高PHB含量的甲烷氧化菌微生态体系，对混合菌进行分离鉴定和生长特性研究。采用纯种分离方法，结合16S rDNA序列分析，菌体间两两交叉划线培养，分离后纯菌的PHB含量及铜离子浓度对混合菌生长进行研究。结果表明，该混合菌群由甲烷氧化菌及其他非甲烷氧化菌构成，甲烷氧化菌属于Methylosinus trichosporium，混合菌中其他微生物构成的伴生菌能充分利用甲烷氧化菌的代谢产物促进生长，并维持菌群结构和功能的稳定。铜离子浓度对混合菌生长有一定影响。该菌群能利用甲烷、甲醇作为生长碳源，长势良好且有MMO活性，可合成PHB，还作为催化剂可以实现丙烯氧化生产环氧丙烷。
위획득고PHB함량적갑완양화균미생태체계，대혼합균진행분리감정화생장특성연구。채용순충분리방법，결합16S rDNA서렬분석，균체간량량교차화선배양，분리후순균적PHB함량급동리자농도대혼합균생장진행연구。결과표명，해혼합균군유갑완양화균급기타비갑완양화균구성，갑완양화균속우Methylosinus trichosporium，혼합균중기타미생물구성적반생균능충분이용갑완양화균적대사산물촉진생장，병유지균군결구화공능적은정。동리자농도대혼합균생장유일정영향。해균군능이용갑완、갑순작위생장탄원，장세량호차유MMO활성，가합성PHB，환작위최화제가이실현병희양화생산배양병완。
In order to obtain Methanotrophs microecological system with high PHB content, the mixed culture are isolated and characterized. The mixed culture growth is studied by pure culture isolation combining with 16S rRNA, cross-scribing, PHB content of each pure strains after separation and different copper ion concentrations. The results show that the mixed culture is composed of methane-oxidizing bacteria and other non-methane-oxidizing bacteria. Methanotrophs belong to Methylosinus trichosporium. The presence of accompanying bacteria in mixed cultures make full use of methanotrophic bacteria metabolites to foster the physiological growth and maintain the stability of structure and function. Copper ion concentration has a certain influence on the growth of the mixed culture. This methane-utilizing mixed culture can not only utilize methane, methanol as carbon source to grew well and has MMO activity, synthesize PHB, also as a catalyst, propylene can be produced to epoxypropane.