农产品加工·学刊(下)
農產品加工·學刊(下)
농산품가공·학간(하)
Academic Periodical of Farm Products Processing
2014年
8期
33-34,37
,共3页
赵金海%杨灵%张志芳%李轶女
趙金海%楊靈%張誌芳%李軼女
조금해%양령%장지방%리질녀
α-淀粉酶%原核表达%酶学性质
α-澱粉酶%原覈錶達%酶學性質
α-정분매%원핵표체%매학성질
α-amylase%procaryotic expression%enzymatic characteristic
α-淀粉酶作为一种十分重要的酶制剂,目前工业上都是以微生物发酵法大规模生产的。从耐高温菌株Geobacillus. POT-5基因组中,用PCR的方法扩增得到该菌的α-淀粉酶基因,此基因被克隆、测序,并在原核Escherichia coli表达系统中进行表达,并对该重组酶的酶学性质进行初步的分析。
α-澱粉酶作為一種十分重要的酶製劑,目前工業上都是以微生物髮酵法大規模生產的。從耐高溫菌株Geobacillus. POT-5基因組中,用PCR的方法擴增得到該菌的α-澱粉酶基因,此基因被剋隆、測序,併在原覈Escherichia coli錶達繫統中進行錶達,併對該重組酶的酶學性質進行初步的分析。
α-정분매작위일충십분중요적매제제,목전공업상도시이미생물발효법대규모생산적。종내고온균주Geobacillus. POT-5기인조중,용PCR적방법확증득도해균적α-정분매기인,차기인피극륭、측서,병재원핵Escherichia coli표체계통중진행표체,병대해중조매적매학성질진행초보적분석。
α-amylase is a very important enzyme, the mass production ofα-amylase is based on microorganism fermentation in the industry at present. The gene ofα-amylases (amyP) is amplified from the genome of the thermostable strain Geobacillus. POT-5 by PCR. This gene is cloned, sequence analysis and expressed in procaryotic expression system in E.coli. This study also develops a preliminary analysis of enzyme properties about the recombinantα-amylase.