CAJ | 학술논문

目的：建立再生障碍性贫血动物模型，检测其碱性成纤维细胞生长因子2(FGF2)的表达。方法 BALB/c小鼠20只分为模型组和对照组，模型组采用5.0 Gy射线照射，随后输注异体小鼠淋巴细胞1×106个，观察小鼠外周血细胞、骨髓单个核细胞计数和骨髓组织切片等指标，ELISA法测定和比较小鼠骨髓单个细胞FGF2蛋白表达。结果模型组小鼠外周血血红蛋白、白细胞、血小板和骨髓单个核细胞数均较对照组小鼠显著降低，差异有统计学意义(P均<0.05)。模型组骨髓单个核细胞FGF2蛋白较对照组亦降低，差异有统计学意义(P<0.05)。结论以射线照射并输注淋巴细胞方法制作的骨髓衰竭小鼠动物模型，其各项指标均符合临床骨髓衰竭的标准，且提示FGF2表达减低参与骨髓衰竭的发生。
목적：건립재생장애성빈혈동물모형，검측기감성성섬유세포생장인자2(FGF2)적표체。방법 BALB/c소서20지분위모형조화대조조，모형조채용5.0 Gy사선조사，수후수주이체소서림파세포1×106개，관찰소서외주혈세포、골수단개핵세포계수화골수조직절편등지표，ELISA법측정화비교소서골수단개세포FGF2단백표체。결과모형조소서외주혈혈홍단백、백세포、혈소판화골수단개핵세포수균교대조조소서현저강저，차이유통계학의의(P균<0.05)。모형조골수단개핵세포FGF2단백교대조조역강저，차이유통계학의의(P<0.05)。결론이사선조사병수주림파세포방법제작적골수쇠갈소서동물모형，기각항지표균부합림상골수쇠갈적표준，차제시FGF2표체감저삼여골수쇠갈적발생。
Objective Previous study found that ifbroblast growth factor-2(FGF2) expression was at a low level in bone marrow of children with aplastic anemia. In this study, we established a bone marrow failure animal model to investigate whether FGF2 is involved in bone marrow failure. Methods BALB/c mice were irradiated by 5.0 Gy ray, and then infused with 1×106 lymphocytes from allogeneic mice lymph node. Peripheral blood cells and bone marrow mononuclear cells counting and bone marrow pathology were done. FGF2 protein in bone marrow mononuclear cells was measured by ELISA. Results Compared with the control group, the counting of hemoglobin, white blood cell, platelet and bone marrow mononuclear cell in aplastic anemia mouse model were signiifcantly deceased (P<0.05). Moreover, FGF2 expression in aplastic anemia mouse model were signiifcantly reduced (P<0.05). Conclusions 5.0 Gy ray irradiation and then 1×106 lymphocyte infusion in mice can induce bone marrow failure similar to the features of aplastic anemia. The low expression of FGF2 in bone marrow of aplastic anemia patients may play an important role in the pathogenesis of aplastic anemia.