CAJ | 학술논문

目的：探讨细菌溶解产物(OM-85BV)对哮喘气道炎症的预防作用及可能机制。方法4~6周龄BALB/c小鼠48只，随机分为6组。a组，空白对照组；b组，OM-85BV对照组；c组，哮喘模型组；d组，地塞米松(Dex)干预组；e组，OM-85BV干预A组；f组，OM-85BV干预B组(干预时间较e组延长10 d)。在实验第1、8和15天分别给c、d、e、f4组小鼠腹腔注射卵清白蛋白(OVA)-Al(OH)3悬液使其致敏，其余组以PBS缓冲液对照。第17~26天，OM-85BV溶于生理盐水(NS)并给f组灌胃，其余5组以生理盐水(NS)灌胃。第27~31天分别给c、d、e和f组OVA的NS溶液滴鼻激发，其余两组以等量NS对照。其中b、e和f组在激发前给予OM-85BV灌胃，d组给予Dex灌胃，其余组用NS对照。末次激发后24 h麻醉处死小鼠，心脏采血测定血清总IgE水平，左肺行肺泡灌洗留取肺泡灌洗液(BALF)进行细胞计数并计算嗜酸性粒细胞(Eos)比例，测定BALF上清液中IFN-γ和IL-4水平，取部分右肺行苏木精-伊红(HE)染色作病理学检查。结果与a、b组比较，c组小鼠支气管痉挛收缩明显，支气管上皮增生紊乱，支气管周围可见以淋巴细胞和嗜酸性细胞为主的炎症细胞浸润，管腔内有少量黏液和脱落的上皮细胞，而且肺泡间隔增宽；BALF中炎性细胞总数、Eos比例增高，BALF上清中IL-4水平增高，而IFN-γ水平降低，血清中总IgE水平增高，差异均有统计学意义(P<0.01)；经干预的d、f组较c组情况明显改善，e组次之，以上各项指标的差异均有统计学意义(P<0.01)。结论口服OM-85BV干预有利于调节Th1/Th2之间的平衡，减轻小鼠哮喘模型气道炎症，可有效预防支气管哮喘气道炎症的发生和发展。
목적：탐토세균용해산물(OM-85BV)대효천기도염증적예방작용급가능궤제。방법4~6주령BALB/c소서48지，수궤분위6조。a조，공백대조조；b조，OM-85BV대조조；c조，효천모형조；d조，지새미송(Dex)간예조；e조，OM-85BV간예A조；f조，OM-85BV간예B조(간예시간교e조연장10 d)。재실험제1、8화15천분별급c、d、e、f4조소서복강주사란청백단백(OVA)-Al(OH)3현액사기치민，기여조이PBS완충액대조。제17~26천，OM-85BV용우생리염수(NS)병급f조관위，기여5조이생리염수(NS)관위。제27~31천분별급c、d、e화f조OVA적NS용액적비격발，기여량조이등량NS대조。기중b、e화f조재격발전급여OM-85BV관위，d조급여Dex관위，기여조용NS대조。말차격발후24 h마취처사소서，심장채혈측정혈청총IgE수평，좌폐행폐포관세류취폐포관세액(BALF)진행세포계수병계산기산성립세포(Eos)비례，측정BALF상청액중IFN-γ화IL-4수평，취부분우폐행소목정-이홍(HE)염색작병이학검사。결과여a、b조비교，c조소서지기관경련수축명현，지기관상피증생문란，지기관주위가견이림파세포화기산성세포위주적염증세포침윤，관강내유소량점액화탈락적상피세포，이차폐포간격증관；BALF중염성세포총수、Eos비례증고，BALF상청중IL-4수평증고，이IFN-γ수평강저，혈청중총IgE수평증고，차이균유통계학의의(P<0.01)；경간예적d、f조교c조정황명현개선，e조차지，이상각항지표적차이균유통계학의의(P<0.01)。결론구복OM-85BV간예유리우조절Th1/Th2지간적평형，감경소서효천모형기도염증，가유효예방지기관효천기도염증적발생화발전。
Objective To establish mouse allergic asthma model and observe the effect of bacterial lysates (OM-85BV) on airway inlfammation. Methods Forty-eight 4 to 6 weeks healthy male BALB/c mice were used as research subjects and randomly divided into six groups, a:control group;b:OM-85BV contral group;c:allergic asthma model;d:dexamethasone group (Dex group);e:OM-85BV A group;f:OM-85BV B group (the intervention time was prolonged 10 days than group e). BALB/c mice were sensitized and challenged with ovalbumin (OVA). Mice in groups c, d, e and f were intraperitoneally administered with antigen (OVA)-Al(OH)3 on days 1, 8 and 15, others were administered by PBS. From the 17th day to the 26th day, Mice in group f were treated with OM-85BV and others were treated with normal saline. In the next days, mice in groups c, d, e and f were intranasal given OVA for 5 consecutive days. Additionally, mice in groups b, e and f were treated with OM-85BV before challenge, while mice in the group d were administered by Dex, others were treated with normal saline at the same dose. Twenty four hours after the last intranasal administration, mice were anesthetized and dissected. Lungs were lavaged with PBS and bronchoalveolar lavage lfuid (BALF) was obtained. The total inlfammatory cells and eosinophils in BALF were counted. The total IgE levels of blood serum and IFN-γ/IL-4 levels of lavage were detected. The removed parts of lung tissue were collected for histological examination. Results Compared with groups a and b, lung tissue biopsies by HE staining from the asthma group showed obvious airway inlfammation. The situation of groups d and f was signiifcantly improved than group c, while the differences between groups e and c were not evident. Total cells and the number of eosinophils in BALF of group c (90.3±13.94×104/ml) were signiifcantly higher than that in groups a and b. Compared with the control group, levels of IL-4 in BALF (119.03±19.92 pg/ml) and IgE in serum (15.86±1.97 ng/ml) increased and levels of IFN-γin BALF (90.50±13.51 pg/ml) reduced signiifcantly. The corresponding levels of groups d, e and f were signiifcantly improved than group c (P<0.01). Conclusions Administered by OM-85BV helps regulate the balance between Th1/Th2 in asthmatic mice, reduce airway inlfammation, and prevent the occurrence and development of airway inlfammation.