南方水产科学
南方水產科學
남방수산과학
SOUTH CHINA FISHERIES SCIENCE
2014年
4期
45-51
,共7页
杨求华%葛辉%方旅平%林琪%何丽斌%周宸
楊求華%葛輝%方旅平%林琪%何麗斌%週宸
양구화%갈휘%방려평%림기%하려빈%주신
刺参%病原菌%塔式弧菌
刺參%病原菌%塔式弧菌
자삼%병원균%탑식호균
Apostichopus japonicus%pathogen%Vibrio tubiashii
2013年春季,福建漳州地区池塘养殖刺参(Apostichopus japonicus)感染不明病原而患病。病参表现出排脏、身体萎缩、体表溃烂等症状,俗称为“腐皮综合征”。为确定引起该病的病原菌,从患病刺参病灶部位分离得到1株优势菌 FJY001,经回接感染试验,证实所分离的细菌为刺参的病原菌。经形态、生理生化、16S rRNA 序列分析和 Biolog 微生物自动鉴定系统等多项指标鉴定,确定该病原菌为塔式弧菌(Vibrio tubiashii)。
2013年春季,福建漳州地區池塘養殖刺參(Apostichopus japonicus)感染不明病原而患病。病參錶現齣排髒、身體萎縮、體錶潰爛等癥狀,俗稱為“腐皮綜閤徵”。為確定引起該病的病原菌,從患病刺參病竈部位分離得到1株優勢菌 FJY001,經迴接感染試驗,證實所分離的細菌為刺參的病原菌。經形態、生理生化、16S rRNA 序列分析和 Biolog 微生物自動鑒定繫統等多項指標鑒定,確定該病原菌為塔式弧菌(Vibrio tubiashii)。
2013년춘계,복건장주지구지당양식자삼(Apostichopus japonicus)감염불명병원이환병。병삼표현출배장、신체위축、체표궤란등증상,속칭위“부피종합정”。위학정인기해병적병원균,종환병자삼병조부위분리득도1주우세균 FJY001,경회접감염시험,증실소분리적세균위자삼적병원균。경형태、생리생화、16S rRNA 서렬분석화 Biolog 미생물자동감정계통등다항지표감정,학정해병원균위탑식호균(Vibrio tubiashii)。
In the spring of 2013,the pond-cultured sea cucumbers(Apostichopus japonicus)were infected by an unrestrained patho-gen in Zhangzhou City of Fujian Province. The sea cucumbers with the disease tentatively termed as“skin ulcerative syndrome”showed symptoms such as viscera ejection,general atrophy and skin ulceration. To determine the bacterial pathogen that caused the disease, one strain(FJY001)was isolated from the lesions of diseased sea cucumbers. Pathogenicity of the strain was confirmed by artificial in-fection and the strain was identified as Vibrio tubiashii by indicators of morphological,physiological and biochemical characteristics as well as sequencing of 16S rRNA and identification of Biolog microbiol system.