CAJ | 학술논문

目的研究肝细胞生长因子(hepatocyte growth factor,HGF)对动脉旁路转流术后吻合口内膜增生的抑制作用.方法 32只新西兰白兔按随机数字表法分为对照组(16只)和实验组(16只),对照组为单纯的静脉移植,实验组术中给予HGF处理(100ng/ml),术后14、28d分别切除移植静脉,HE染色、弹力纤维染色、计算机图像分析系统测量和计算内膜厚度及中膜厚度、内膜/中膜厚度比值(I/M),免疫组织化学方法检测静脉移植物平滑肌细胞增殖细胞核抗(PCNA)的表达.结果对照组静脉移植术后14d I/M(0.81 ±0.05)高于实验组(0.47±0.05),差异有统计学意义(P＜0.01),对照组静脉移植术后28d I/M(0.73 ±0.01)高于实验组(0.65 ±0.01),差异有统计学意义(P＜0.01).实验组静脉移植术后的PCNA指数明显低于对照组(P＜0.01).结论肝细胞生长因子对动脉旁路转流术后吻合口内膜增生有抑制作用.
목적 연구간세포생장인자(hepatocyte growth factor,HGF)대동맥방로전류술후문합구내막증생적억제작용.방법 32지신서란백토안수궤수자표법분위대조조(16지)화실험조(16지),대조조위단순적정맥이식,실험조술중급여HGF처리(100ng/ml),술후14、28d분별절제이식정맥,HE염색、탄력섬유염색、계산궤도상분석계통측량화계산내막후도급중막후도、내막/중막후도비치(I/M),면역조직화학방법검측정맥이식물평활기세포증식세포핵항(PCNA)적표체.결과 대조조정맥이식술후14d I/M(0.81 ±0.05)고우실험조(0.47±0.05),차이유통계학의의(P＜0.01),대조조정맥이식술후28d I/M(0.73 ±0.01)고우실험조(0.65 ±0.01),차이유통계학의의(P＜0.01).실험조정맥이식술후적PCNA지수명현저우대조조(P＜0.01).결론 간세포생장인자대동맥방로전류술후문합구내막증생유억제작용.
Objective Study of the effects of hepatocyte growth factor on inhibit Intimal hyperplasia of the anastomotic stoma after carotid artery bypass grafting.Methods Thirty-two New Zealand white rabbits were randomly divided into control group and the experimental group.The veins were pretreated with saline solution(control group)only or pretreated with HGF(experimental group ;100ng/ml).The vein grafts were harvested at 14 days,28days after operation,HE Stain and Elastic fibrin Stain,The thickness of Intima and media in the vein grafts,intima-media ratio(I/M) was calculated by computer image analysis system.PCNA Immunohistochemistry was performed.Results The thickness of Intima and media in the vein grafts of control group surpassed experimental group significantly(P ＜0.01).At 14d I/M in the vein grafts of control group (0.81 ± 0.05) surpassed experimental group (0.47 ± 0.05) (P ＜ 0.01),At 28d I/M in the vein grafts of control group(0.73 ± 0.01)surpassed experimental group (0.65 ± 0.01) (P ＜ 0.01).The vascular smooth muscle cell proliferation in experimental group was significantly lower than that in control group (P ＜ 0.01).Conclusion Treatment of veins grafts with HGF can significantly inhibit intimal hyperplasia in a rabbit carotid artery bypass grafting model.