CAJ | 학술논문

以化学共沉淀法合成Fe3O4纳米粒子为磁核，采用乳化交联法制备磁性壳聚糖微球，并对其形貌、结构和磁饱和强度等性质进行了表征。以磁性壳聚糖微球作为载体，固定化猪肺粗提物中的血管紧张素转化酶，并对固定化条件进行研究。结果表明，固定化血管紧张素转化酶的最佳条件为：pH值为8.3，最佳温度为50℃，最佳时间为1.5 h，最佳酶溶液蛋白浓度为6 mg/mL，此时固定化酶活力最高为0.048 U/g微球。与游离酶相比，固定化酶的pH值稳定性和热稳定性均得到提高。固定化酶重复使用10次，仍然保持40%以上相对活力，说明磁性壳聚糖微球是固定化血管紧张素转化酶的良好载体。
이화학공침정법합성Fe3O4납미입자위자핵，채용유화교련법제비자성각취당미구，병대기형모、결구화자포화강도등성질진행료표정。이자성각취당미구작위재체，고정화저폐조제물중적혈관긴장소전화매，병대고정화조건진행연구。결과표명，고정화혈관긴장소전화매적최가조건위：pH치위8.3，최가온도위50℃，최가시간위1.5 h，최가매용액단백농도위6 mg/mL，차시고정화매활력최고위0.048 U/g미구。여유리매상비，고정화매적pH치은정성화열은정성균득도제고。고정화매중복사용10차，잉연보지40%이상상대활력，설명자성각취당미구시고정화혈관긴장소전화매적량호재체。
Fe3O4 magnetic nanoparticle was prepared by chemical co-precipitation method,and magnetic chitosan microsphere was prepared through crosslink emulsification process applying Fe3O4 magnetic nanoparticle as magnetic nuclear. Also,the properties of magnetic composite microsphere were characterized. Crude angiotensin converting enzyme(ACE) extracted from hog lungs was immobilized on magnetic chitosan microspheres , factors influencing the immobilization were investigated. The experimental results showed that the optimal conditions for preparation immobilized ACE were immobilization pH=8.3,temperature 50 ℃,time 1.5 h and protein concentration of ACE 6 mg/mL,with a optimal specific activity of 0.048 U/g spheres. Compared with free ACE,pH and thermal stability of immobilized ACE were increased. After 10 repetitive operations,the activity of immobilized ACE remained more than 40%of its initial activity. These results indicated that magnetic chitosan microsphere can be a kind of good support for immobilizing ACE.