CAJ | 학술논문

目的研究肝再生磷酸酶3（PRL-3）在胶质瘤细胞SHG-44中作用。方法采用siRNA干涉的方法下调SHG-44中PRL-3表达水平，通过Western blot方法检测干涉效果，绘制细胞生长曲线、流式细胞仪检测细胞周期及凋亡以观察下调PRL-3对SHG-44细胞的影响。结果 Western blot提示siRNA能够下调PRL-3在SHG-44细胞中的表达，细胞生长曲线显示下调PRL-3能够抑制细胞的增殖，流式细胞仪检测发现下调PRL-3的表达可以促进SHG-44细胞G1期阻滞，S期减少，凋亡增加。结论下调PRL-3能够明显抑制SHG-44细胞的增殖，PRL-3很可能成为脑胶质瘤基因治疗的新靶点。
목적연구간재생린산매3（PRL-3）재효질류세포SHG-44중작용。방법채용siRNA간섭적방법하조SHG-44중PRL-3표체수평，통과Western blot방법검측간섭효과，회제세포생장곡선、류식세포의검측세포주기급조망이관찰하조PRL-3대SHG-44세포적영향。결과 Western blot제시siRNA능구하조PRL-3재SHG-44세포중적표체，세포생장곡선현시하조PRL-3능구억제세포적증식，류식세포의검측발현하조PRL-3적표체가이촉진SHG-44세포G1기조체，S기감소，조망증가。결론하조PRL-3능구명현억제SHG-44세포적증식，PRL-3흔가능성위뇌효질류기인치료적신파점。
Objective To investigate the inhibition of phosphatase of regenerating liver-3(PRL-3)in brain glioma cell line-SHG-44 .Methods PRL-3 siRNA was designed and transfected into SHG-44 cell ( the transfection was performed using Lipofectamine TM 2000 ) .The interfering effects were detected by western blot .The affection of the siRNA on cell proliferation was observed through cell growth curve .The variation of cell cycle and apoptosis were detected by flow cytometry .Results The results of RT-PCR and western blot indicated that the siRNA could down-regulate the expression of PRL-3 in SHG-44 cell properly .Cell growth curves indicated that down-regulation of PRL-3 could suppress the proliferation of the SHG-44 cells.siRNA-transfected cells showed a increase in G 1 phase and a decrease in S phase .PRL-3 siRNA induced apoptosis increasing in comparison with the control .Conclusion Down-regulation of PRL-3 could suppress the proliferation of the SHG-44 cells significantly .These results indicated that PRL-3 might become a new target gene for glioma therapy .