CAJ | 학술논문

目的观察不同剂量的姜黄素在体外对肝癌细胞Bel7402、QGY7703生长的影响，探讨姜黄素抑制肝癌细胞增殖、促进其凋亡的可能分子学机制。方法 MTT 法检测不同浓度姜黄素对 Bel7402、QGY7703细胞增殖的抑制作用，流式细胞仪分析细胞的凋亡率，Western Blot 法检测姜黄素对Wnt通路中β-catenin蛋白表达的影响，PCR法检测下游靶基因c-myc、VEGF、cyclinD1 mRNA在不同浓度姜黄素作用后表达情况的变化。结果 MTT实验显示：经浓度为5、10、20、40、80μmol/L的姜黄素作用48 h后，肝癌细胞Bel7402、QGY7703的增殖明显受到抑制，抑制率与阴性对照组比较有统计学意义（ P＜0.05）。药物浓度越大，增殖抑制率越明显，呈剂量依赖性（ r＝0.9626，r＝0.9720）。 FCM分析显示：实验组肝癌细胞凋亡率均高于阴性对照组（P＜0.05），且随着药物浓度的增加，细胞凋亡率逐渐升高，呈剂量依赖性（r＝0.9646，r＝0.9949）。 Western Blot结果显示：经姜黄素作用48 h后的Bel7402和QGY7703细胞，其胞质β-catenin蛋白含量低于阴性对照组，除10μmol/L浓度组外，20、40、80μmol/L浓度组与阴性对照组比较均有统计学意义（ P＜0.05）。药物浓度越大，胞质β-catenin蛋白含量越少，呈剂量依赖性（ r＝-0.9594，r＝-0.9488）。 PCR结果显示：经姜黄素作用后的肝癌细胞，c-myc、VEGF、cyclinD1 mRNA表达量较阴性对照组均有所减少（ P＜0.05）。结论姜黄素能够有效地抑制肝癌细胞的增殖并促进其凋亡，其机制可能与姜黄素下调经典Wnt/β-catenin信号通路有关。
목적관찰불동제량적강황소재체외대간암세포Bel7402、QGY7703생장적영향，탐토강황소억제간암세포증식、촉진기조망적가능분자학궤제。방법 MTT 법검측불동농도강황소대 Bel7402、QGY7703세포증식적억제작용，류식세포의분석세포적조망솔，Western Blot 법검측강황소대Wnt통로중β-catenin단백표체적영향，PCR법검측하유파기인c-myc、VEGF、cyclinD1 mRNA재불동농도강황소작용후표체정황적변화。결과 MTT실험현시：경농도위5、10、20、40、80μmol/L적강황소작용48 h후，간암세포Bel7402、QGY7703적증식명현수도억제，억제솔여음성대조조비교유통계학의의（ P＜0.05）。약물농도월대，증식억제솔월명현，정제량의뢰성（ r＝0.9626，r＝0.9720）。 FCM분석현시：실험조간암세포조망솔균고우음성대조조（P＜0.05），차수착약물농도적증가，세포조망솔축점승고，정제량의뢰성（r＝0.9646，r＝0.9949）。 Western Blot결과현시：경강황소작용48 h후적Bel7402화QGY7703세포，기포질β-catenin단백함량저우음성대조조，제10μmol/L농도조외，20、40、80μmol/L농도조여음성대조조비교균유통계학의의（ P＜0.05）。약물농도월대，포질β-catenin단백함량월소，정제량의뢰성（ r＝-0.9594，r＝-0.9488）。 PCR결과현시：경강황소작용후적간암세포，c-myc、VEGF、cyclinD1 mRNA표체량교음성대조조균유소감소（ P＜0.05）。결론강황소능구유효지억제간암세포적증식병촉진기조망，기궤제가능여강황소하조경전Wnt/β-catenin신호통로유관。
Objective To investigate the effect of different concentrations of curcumin on inhibiting the proliferation of hepatocellular carcinoma , and then to explore the possible molecular mechanism of inhibiting the proliferation and inducing apoptosis of hepatocellular carcinoma cells .Methods MTT assay was used to detect the growth of cell .The flow cytometric analysis was performed to evaluate the cell apoptosis .Western Blot was applied to test the level of β-catenin.PCR was used to detect the expression of downstream target genes ,such as c-myc,VEGF, cyclinD1.Results When compared with the negative control group , results from MTT assay showed that as the concentration of curcumin increased from 5 μmol/L to 80 μmol/L, inhibition of Bel7402 and QGY7703 cells proliferation increased ( P<0.05 ) ,as well in a dose-dependent manner ( r=0.9626 ,r=0.9720 ) .The FCM analysis showed that after the intervention of curcumin in Bel 7402,QGY7703 cells for 48 hours,the percentage of apoptotic cells were significantly more than the control group without curcumin to deal with ( P <0.05 ) .The more concentrations ,the greater apoptosis rate ( r=0.9646 , r =0.9949 ) .The result of Western Blot indicated that the intracellular core protein expression of β-catenin levels in the experimental group cells were significantly lower than the negative control group (P<0.05).In Bel7402 and QGY7703 cells,except 10 μmol/L of curcumin,compared with the control group , other concentration groups all had statistically significant ( P <0.05 ) .The more concentrations,the less amount of expression of β-catenin(r=-0.9594,r=-0.9488).The result of PCR indicated that the expression of c-myc, VEGF, cyclinD1 mRNA was significantly lower than the negative control group ( P<0.05 ) .Conclusion Curcumin effectively inhibits the growth and induces apoptosis of human hepatoma carcinoma cell lines,Bel7402 and QGY7703,maybe through down-regulating the canonical Wnt/β-catenin signaling pathway .