CAJ | 학술논문

目的观察中药助孕方对胚泡着床障碍模型小鼠着床窗期子宫内膜胞饮突及白血病抑制因子（ LIF）表达的影响。方法选择6～8周龄昆明小鼠，交配后，将妊娠小鼠随机分成正常组、着床障碍组、着床障碍＋中药组和中药组四组。采用扫描电镜观察各组小鼠在妊娠第4天晚22时（第1时间点，T1）和妊娠第5天上午10时（第2时间点，T2），共两个时间点子宫内膜胞饮突的表达，并采用免疫组化和Real Time-PCR技术观察各组小鼠着床窗期LIF的原位表达和其mRNA的表达。结果正常组T1时子宫内膜表面表达大量发育中的胞饮突， T2时表达大量发育完全的胞饮突，与正常组相比，着床障碍组表达受抑制，着床障碍＋中药组胞饮突的表达接近于正常组；着床障碍组小鼠着床窗期的LIF的蛋白表达和mRNA表达较正常组有明显的降低（0.23±0.02 vs．0.40±0.07，P＜0.05），着床障碍＋中药组的表达与模型组相比显著增加（0.39±0.02 vs．0.23±0.02，P＜0.05），接近正常组水平。在上述指标中中药组与对照组相比无统计学差异（ P＞0.05）。结论中药助孕方能使着床窗期着床障碍模型的胞饮突表达同步并增加子宫内膜LIF的表达，改善小鼠的子宫内膜容受性。
목적관찰중약조잉방대배포착상장애모형소서착상창기자궁내막포음돌급백혈병억제인자（ LIF）표체적영향。방법선택6～8주령곤명소서，교배후，장임신소서수궤분성정상조、착상장애조、착상장애＋중약조화중약조사조。채용소묘전경관찰각조소서재임신제4천만22시（제1시간점，T1）화임신제5천상오10시（제2시간점，T2），공량개시간점자궁내막포음돌적표체，병채용면역조화화Real Time-PCR기술관찰각조소서착상창기LIF적원위표체화기mRNA적표체。결과정상조T1시자궁내막표면표체대량발육중적포음돌， T2시표체대량발육완전적포음돌，여정상조상비，착상장애조표체수억제，착상장애＋중약조포음돌적표체접근우정상조；착상장애조소서착상창기적LIF적단백표체화mRNA표체교정상조유명현적강저（0.23±0.02 vs．0.40±0.07，P＜0.05），착상장애＋중약조적표체여모형조상비현저증가（0.39±0.02 vs．0.23±0.02，P＜0.05），접근정상조수평。재상술지표중중약조여대조조상비무통계학차이（ P＞0.05）。결론중약조잉방능사착상창기착상장애모형적포음돌표체동보병증가자궁내막LIF적표체，개선소서적자궁내막용수성。
Objective To observe the effect of Zhuyun recipe ( ZYF ) on the expression of endometrial pinopodes and leukemia inhibitory factor ( LIF ) during the implantation window in blastocyst implantation dysfunctional(BID) mice.Methods 6-8 weeks pregnant Kunming mice were randomly assigned to the control group,BID group,BID+ZYF group and ZYF only group postcoitally .The expression of endometrial pinopodes at 22∶00 on Pd4( T1 ) and at 10∶00 on Pd5 ( T2 ) was determined by scanning electron microscopy .LIF protein and its mRNA were detected with immunohistochemistry and Real Time-PCR analyses.Results In control group,abundant developing pinopodes were expressed in endometrial surface at T 1, whereas abundant fully developed pinopodes expressed at T2.Compared with control group ,the expression in BID group was restrained ,whereas BID+ZYF group was closed to the control group .The expression of LIF and its mRNA was a significant decrease in BID group compared with control group , whereas BID +ZYF group was increased .No significant difference was found in the measured indices between the ZYF only group and control group .Conclusions ZYF synchronize the expression of pinopodes and increased the expression of LIF in the BID group ,to improve endometrial receptivity .