中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
11期
4867-4872
,共6页
周美汛%刘亚敏%孔天翰%董伟华
週美汛%劉亞敏%孔天翰%董偉華
주미신%류아민%공천한%동위화
蝎毒液类%肽类%间质干细胞%细胞增殖%辐射防护
蝎毒液類%肽類%間質榦細胞%細胞增殖%輻射防護
갈독액류%태류%간질간세포%세포증식%복사방호
Scorpion venoms%Peptides%Mesenchymal stem cells%Cell proliferation%Radiation pro-tection
目的观察蝎毒增殖多肽（ Buthus martensii Karsch proliferation peptide ，BmKpp）对X射线辐射后大鼠骨髓间充质干细胞（rat bone-marrow-derived mesenchymal stem cells ，rBMSCs）生长的影响。方法利用全骨髓细胞贴壁法分离纯化rBMSCs，体外扩增传代，取第3代至第8代细胞为实验对象。流式细胞术对rBMSCs进行细胞表型鉴定后，将细胞设为非辐射组（ IR-组）和辐射组（ IR＋组，总剂量为8.0 Gy、标称剂量率300 MU/min的X射线照射）。 CCK-8法检测BmKpp对各组细胞在不同时间的增殖情况。结果体外培养的第3代或第8代rBMSCs 的细胞表型均为：CD29＋/CD90＋/CD34-/CD45-。在BmKpp对X射线辐射后rBMSCs作用的最低有效浓度筛选的实验中，BmKpp 8μg/ml处理组的吸光度（ A值）显著高于辐射对照组（ P＜0.01）；BmKpp（8μg/ml）有促进辐射后的rBMSCs增殖的作用，尤以辐射后第8天、第16天、第18天和第20天效果最为明显，其A值与对照组的相比，P均＜0.05；BmKpp（8μg/ml）未能显著促进非辐射rBMSCs的增殖，与对照组相比无统计学差异（ P＞0.05）。结论 BmKpp能促进辐射后的rBMSCs的增殖，从而发挥其促进辐射后造血功能的恢复作用。
目的觀察蝎毒增殖多肽（ Buthus martensii Karsch proliferation peptide ，BmKpp）對X射線輻射後大鼠骨髓間充質榦細胞（rat bone-marrow-derived mesenchymal stem cells ，rBMSCs）生長的影響。方法利用全骨髓細胞貼壁法分離純化rBMSCs，體外擴增傳代，取第3代至第8代細胞為實驗對象。流式細胞術對rBMSCs進行細胞錶型鑒定後，將細胞設為非輻射組（ IR-組）和輻射組（ IR＋組，總劑量為8.0 Gy、標稱劑量率300 MU/min的X射線照射）。 CCK-8法檢測BmKpp對各組細胞在不同時間的增殖情況。結果體外培養的第3代或第8代rBMSCs 的細胞錶型均為：CD29＋/CD90＋/CD34-/CD45-。在BmKpp對X射線輻射後rBMSCs作用的最低有效濃度篩選的實驗中，BmKpp 8μg/ml處理組的吸光度（ A值）顯著高于輻射對照組（ P＜0.01）；BmKpp（8μg/ml）有促進輻射後的rBMSCs增殖的作用，尤以輻射後第8天、第16天、第18天和第20天效果最為明顯，其A值與對照組的相比，P均＜0.05；BmKpp（8μg/ml）未能顯著促進非輻射rBMSCs的增殖，與對照組相比無統計學差異（ P＞0.05）。結論 BmKpp能促進輻射後的rBMSCs的增殖，從而髮揮其促進輻射後造血功能的恢複作用。
목적관찰갈독증식다태（ Buthus martensii Karsch proliferation peptide ，BmKpp）대X사선복사후대서골수간충질간세포（rat bone-marrow-derived mesenchymal stem cells ，rBMSCs）생장적영향。방법이용전골수세포첩벽법분리순화rBMSCs，체외확증전대，취제3대지제8대세포위실험대상。류식세포술대rBMSCs진행세포표형감정후，장세포설위비복사조（ IR-조）화복사조（ IR＋조，총제량위8.0 Gy、표칭제량솔300 MU/min적X사선조사）。 CCK-8법검측BmKpp대각조세포재불동시간적증식정황。결과체외배양적제3대혹제8대rBMSCs 적세포표형균위：CD29＋/CD90＋/CD34-/CD45-。재BmKpp대X사선복사후rBMSCs작용적최저유효농도사선적실험중，BmKpp 8μg/ml처리조적흡광도（ A치）현저고우복사대조조（ P＜0.01）；BmKpp（8μg/ml）유촉진복사후적rBMSCs증식적작용，우이복사후제8천、제16천、제18천화제20천효과최위명현，기A치여대조조적상비，P균＜0.05；BmKpp（8μg/ml）미능현저촉진비복사rBMSCs적증식，여대조조상비무통계학차이（ P＞0.05）。결론 BmKpp능촉진복사후적rBMSCs적증식，종이발휘기촉진복사후조혈공능적회복작용。
Objective To study the effect of Buthus martensii Karsch proliferation peptide ( BmKpp) on rat bone-marrow-derived mesenchymal stem cells ( rBMSCs ) growth after X-ray radiation.Methods rBMSCs were isolated from the whole bone marrow and cultured in vivo.Take P3-P8 cells for experimental subject .Cellular immune phenotype was identified by flow cytomertry .rBMSCs were divided into two experimental groups:normal group ( non-radiation group ,IR-) ,and irradiation group ( IR+) which rBMSCs were exposed to X-ray for 8.0 Gy total dose and nominal dose rate 300 MU/min with SAD method .Each experimental group was composed of control group and BmKpp treated groups .CCK-8 method was used to examine the proliferation of rBMSCs of different groups at different times.Results The cellular immune phenotype identification of P 3-P8 cells was showed as CD29 +/CD90 +/CD34 -/CD45 -.The absorbance value of 8 μg/ml BmKpp treated group was higher significantly than that of control group(P<0.01).BmKpp(8 μg/ml)could promote irradiated rBMSCs proliferation effectively ,especially at the 8th day,16th day,18th and 20th day after irradiation(P <0.05).But BmKpp(8 μg/ml)could not promoting the proliferation of non-irradiated rBMSCs .Conclusions BmKpp could promote X-ray irradiated rBMSCs proliferation , but has no obvious effect on normal rBMSCs .It indicated that BmKpp promote the proliferation of rBMSCs , which need to be in a certain stress conditions ( eg.irradiation ) , may be related to the recovery of hematopoietic function after irradiation.
