中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
11期
4844-4848
,共5页
彭紫元%纪梓良%张琼丽%段永刚%镇万华%唐爱发%蔡志明
彭紫元%紀梓良%張瓊麗%段永剛%鎮萬華%唐愛髮%蔡誌明
팽자원%기재량%장경려%단영강%진만화%당애발%채지명
膀胱肿瘤%T淋巴细胞,调节性%免疫抑制%T淋巴细胞亚群%细胞因子类
膀胱腫瘤%T淋巴細胞,調節性%免疫抑製%T淋巴細胞亞群%細胞因子類
방광종류%T림파세포,조절성%면역억제%T림파세포아군%세포인자류
Urinary bladder neoplasms%T-Lymphocytes,regulatory%Immune suppression%T-lymphocyte subsets%Cytokines
目的研究调节性T细胞在膀胱癌患者组织和血液中的表达及其临床意义。方法收集膀胱癌患者组织标本及其血液标本各20例,应用流式细胞术检测T淋巴细胞亚群的分布,实时定量PCR技术检测膀胱癌组织及癌旁组织细胞因子转化生长因子-β( TGF-β),白介素( IL )-4、-10、-12、-17和干扰素-γ( IFN-γ)等表达水平,并分析调节性T细胞的表达与膀胱癌临床病理特征的关系。结果与膀胱癌癌旁组织标本相比(3.58±0.31)%,在膀胱癌中调节性T细胞显著增多(29.10±0.75)%(P<0.01),浸润性膀胱癌组织中CD4+CD25+Treg细胞的数量与肿瘤分级和分期具有显著相关性(r=0.73,P<0.05),而且抑制性细胞因子TGF-β和IL-10的表达量显著增多(P<0.01),IL-17表达亦增多(P<0.01)。与健康对照组(5.2±0.63)%相比,膀胱癌患者外周血中调节性T细胞亦显著增多(15.68±0.54)%,且膀胱癌组织中的调节性T细胞阳性细胞百分比较外周血中更多( P<0.01)。结论膀胱癌患者组织及外周血均表现免疫抑制状态,膀胱癌组织的调节性T细胞阳性表达数量与临床病理特征具有相关性。
目的研究調節性T細胞在膀胱癌患者組織和血液中的錶達及其臨床意義。方法收集膀胱癌患者組織標本及其血液標本各20例,應用流式細胞術檢測T淋巴細胞亞群的分佈,實時定量PCR技術檢測膀胱癌組織及癌徬組織細胞因子轉化生長因子-β( TGF-β),白介素( IL )-4、-10、-12、-17和榦擾素-γ( IFN-γ)等錶達水平,併分析調節性T細胞的錶達與膀胱癌臨床病理特徵的關繫。結果與膀胱癌癌徬組織標本相比(3.58±0.31)%,在膀胱癌中調節性T細胞顯著增多(29.10±0.75)%(P<0.01),浸潤性膀胱癌組織中CD4+CD25+Treg細胞的數量與腫瘤分級和分期具有顯著相關性(r=0.73,P<0.05),而且抑製性細胞因子TGF-β和IL-10的錶達量顯著增多(P<0.01),IL-17錶達亦增多(P<0.01)。與健康對照組(5.2±0.63)%相比,膀胱癌患者外週血中調節性T細胞亦顯著增多(15.68±0.54)%,且膀胱癌組織中的調節性T細胞暘性細胞百分比較外週血中更多( P<0.01)。結論膀胱癌患者組織及外週血均錶現免疫抑製狀態,膀胱癌組織的調節性T細胞暘性錶達數量與臨床病理特徵具有相關性。
목적연구조절성T세포재방광암환자조직화혈액중적표체급기림상의의。방법수집방광암환자조직표본급기혈액표본각20례,응용류식세포술검측T림파세포아군적분포,실시정량PCR기술검측방광암조직급암방조직세포인자전화생장인자-β( TGF-β),백개소( IL )-4、-10、-12、-17화간우소-γ( IFN-γ)등표체수평,병분석조절성T세포적표체여방광암림상병리특정적관계。결과여방광암암방조직표본상비(3.58±0.31)%,재방광암중조절성T세포현저증다(29.10±0.75)%(P<0.01),침윤성방광암조직중CD4+CD25+Treg세포적수량여종류분급화분기구유현저상관성(r=0.73,P<0.05),이차억제성세포인자TGF-β화IL-10적표체량현저증다(P<0.01),IL-17표체역증다(P<0.01)。여건강대조조(5.2±0.63)%상비,방광암환자외주혈중조절성T세포역현저증다(15.68±0.54)%,차방광암조직중적조절성T세포양성세포백분비교외주혈중경다( P<0.01)。결론방광암환자조직급외주혈균표현면역억제상태,방광암조직적조절성T세포양성표체수량여림상병리특정구유상관성。
Objective To investigate the expression of regulatory T cells ( Treg cells ) in bladder carcinoma and patients′blood and its clinical significance .Methods The tissue and blood samples were collected from patients with bladder cancer(n=20).T lymphocyte subsets were detected by flow cytometer and cytokines (TGF-β,IL-4,IL-10,IL-12,IL-17 and IFN-γ) in cancerous and paracancerous tissues were assessed by real-time PCR technology. Clinical pathologic factors were analyzed accordingly .Results A significant increase of the percentage of Treg cells was found in the bladder carcinoma compared with paracancerous tissues [ ( 29.10 ±0.75 )%;P <0.01 ] , and inhibitory cytokines ( TGF-βand IL-10 ) increased significantly in bladder carcinoma with a rise of IL-17.The percentage of Blood Treg cells increased significantly in carcinoma group compared with normal group but less than that of cancerous tissues in bladder carcinoma [ ( 15.68 ±0.54 )%;P<0.01 ] .Conclusion The overexpression of Treg cells from bladder carcinoma patients indicates an immunosuppressive regulatory profile and the percentage of cancerous Treg cells are associated with the clinical pathologic factors in bladder carcinoma .